The oral bacterium Porphyromonas gingivalis is a key etiological agent of human periodontitis, a prevalent chronic disease that affects up to 80% of the adult population worldwide. P. gingivalis exhibits neuraminidase activity. However, the enzyme responsible for this activity, its biochemical features, and its role in the physiology and virulence of P. gingivalis remain elusive. In this report, we found that P. gingivalis encodes a neuraminidase, PG0352 (Sia Pg ). Transcriptional analysis showed that PG0352 is monocistronic and is regulated by a sigma 70 -like promoter. Biochemical analyses demonstrated that Sia Pg is an exo-␣-neuraminidase that cleaves glycosidic-linked sialic acids. Cryoelectron microscopy and tomography analyses revealed that the PG0352 deletion mutant (⌬PG352) failed to produce an intact capsule layer. Compared to the wild type, in vitro studies showed that ⌬PG352 formed less biofilm and was less resistant to killing by the host complement. In vivo studies showed that while the wild type caused a spreading type of infection that affected multiple organs and all infected mice were killed, ⌬PG352 only caused localized infection and all animals survived. Taken together, these results demonstrate that Sia Pg is an important virulence factor that contributes to the biofilm formation, capsule biosynthesis, and pathogenicity of P. gingivalis, and it can potentially serve as a new target for developing therapeutic agents against P. gingivalis infection.
Breast milk is the most important food for babies, especially in the first month of baby life. The lack of production of breast milk after childbirth can be caused by a lack of stimulation of the hormone prolactin and oxytocin. Oxytocin massage is one solution to overcome the non-smooth production of breast milk. The use of lavender essential oil can help mothers to improve relaxation and comfort to increase the production of breast milk. The study aimed to determine the effect of oxytocin massage method using lavender essential oil on the smooth production of breast milk in postpartum mothers in Rejang Lebong Regency. The research hypothesis is that there is an effect of oxytocin massage method using lavender essential oil on the smooth production of breast milk in postpartum mothers. The population in this study were postpartum mothers on day 2. The sample was 32 people of two groups. The instruments used were questionnaires and observation sheets for the action of oxytocin massage method using lavender essential oil. Quantitative research method with quasi-experimental design, with pre-test and post-test design with control group a research design that aims to test the causal relationship.In this study, the intervention of oxytocin massage method will be used using lavender essential oil for the smooth production of breast milk in postpartum mothers. The results showed that there was an effect of oxytocin massage method using lavender essential oil on the smooth production of breast milk in postpartum mothers with a P <0.05. Conclusion: There is an effect of oxytocin massage using lavender essential oil on the smooth production of breast milk.
Backgroud: Self-care education can be carried out patients with diabetes through the Diabetes Self-Management Education (DSME) method. The DSME method helps people to perform with type 2 diabetes self-management in managing and treating type 2 diabetes, such as controlling blood sugar levels and HbA1c values. Objective: The purpose of this research is to understand the effect of DSME for reducing blood sugar levels and decreasing HbA1c values in type 2 diabetes patients. Methods: The design of this research is a Pre-Experimental with Pretest-Posttest Group Design with a Pretest-Posttest Group Design, approach which aims to see the use of the DSME method for reducing blood sugar levels and decreasing HbA1c values in type 2 diabetes patients. This research was conducted from July to October 2021. The research location was at the Curup Regional General Hospital, Rejang Lebong Regency. Respondents in this study were 35 patients with type 2 diabetes. The instrument used was the DSME method consisting of 3 instruments, including the theoretical concept of diabetes, care and treatment of diabetes, and complications of diabetes. Results: The results of the study show a significant change in blood sugar levels before and after the DSME. Before being carried out, blood sugar levels were with a Mean±SD of 341.60±157.589; meanwhile, after the intervention, blood sugar levels became 107.97±9.984 with a p:0.001. On the other hand, the HbA1c Value before intervention was Mean±SD 8.4313±2.2610, and after the intervention, the HbA1c value became 6.4553±1.2426 with p=0.001. Conclusion: DSME can be used as an alternative intervention that decreases in blood sugar levels and HbA1c values in patients with type 2 diabetes.
Chronic kidney failure is a world health problem because it is difficult to cure; the cost of treatment and treatment is expensive. Patients with chronic renal failure undergoing hemodialysis are unable to maintain the balance of metabolism and electrolyte fluid, causing an increase in uremia in creatinine in the blood. Patients with chronic renal failure undergoing extended hemodialysis will affect the decline in quality of life. This study aims to determine the effect of psychoeducation on creatinine levels of urea in the blood and the quality of life of patients with chronic renal failure undergoing hemodialysis at Curup General Hospital. Methods: This study uses a pre-experimental method with a research design using one group pretest-posttest design. Patients with chronic renal failure were given psychoeducation for six weeks and then measured the levels of urea and creatinine in the blood and measured the quality of life of patients with chronic renal failure undergoing hemodialysis at Curup General Hospital. The number of respondents in this study was 35 patients. The Wilcoxon nonparametric test analyzed data. Results: After psychoeducation, there were significant differences in creatinine urea levels in the blood and changes in the quality of life of patients with chronic renal failure undergoing hemodialysis at the Curup General Hospital. Conclusion: Psychoeducation affects the levels of urea and creatinine in the blood and changes in the quality of life of patients with chronic renal failure undergoing hemodialysis.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.