A new route of in vitro mass propagation protocol of Gerbera jamesonii (H. Bolus ex Bolus f.) derived from application of thin cell layers (TCL) and adenine sulphate (AS) was successfully developed and established. Shoot tip explants and half-strength MS medium containing 0.25 mg/l N6-benzylaminopurine (BAP), 20 g/l sucrose and 7 g/l Swallow agar were used as explant source and basic medium. Different TCL of transversal TCL (tTCL) and longitudinal TCL (lTCL) in four slicing positions of 1, 2, 3 and 4; varieties and clones i.e. G. jamesonii ‘Black Jack’, ‘Carambole’, ‘Nuance’, ‘Violente’, 01.098 and 11.46 clone; AS concentrations viz. 0, 20, 40, 60, 80 and 100 mg/l were tested in the study. Each step of in vitro culture established had unique and specific results. In the initiation stage, first slicing position of ‘Black Jack’ shoot tip tTCL was the most optimal combination treatment to produce 7.0 shoots per explant with 13.5 leaves. The first slicing position on shoot tip explants of 01.098 clone tTCL and 20 mg/l AS in half-strength MS medium containing 0.25 mg/l BAP were the most optimal combination treatment in obtaining the highest number of shoots produced per shoot up to 9.4 shoots per shoot with 34.1 leaves and 2.37 cm length of leaves in the proliferation stage, however the treatment did not give significant effect compared to control. Under periodical subcultures on the basic medium, number of shoots and leaves increased gradually from the initial culture with 3-6 shoots per shoot and 9.4-11.6 leaves till the fourth subculture with 6-11 shoots per shoot and 16.7-28.8 leaves and declined thereafter. Subculturing of shoots in accordance to produce qualified shoots for planting materials could be carried out till sixth to seventh subculture. The highest shoot multiplication rate (SMR) was established on 01.098 clone with as high as 7.3. The well shoots were easily rooted on half-strength MS medium supplemented with 0.1 mg/l BAP, 0.05 mg/l NAA and 1.5 g/l AC. Plantlets were then transferred to ex vitro condition for acclimatization on a mixture of burned-rice husk and organic manure (1:1, v/v) with 85-100% survivability. The ‘Black Jack’ and 11.46 clone were the best genotypes on the acclimatization stage with 100% survivability of plantlets. Results of the study have implication that first slicing position of shoot tip tTCL can be applied in establishing of in vitro propagation protocol for other gerberas.
A new route of in vitro propagation of gerbera selected clones was successfully established using young capitula in tight buds and buds that were started to unfold stage as explant source. The one-fourth pieces of young capitula of tight flower stage and half-strength MS medium containing 0.25 mg/l BAP was the suitable for initiation and produced higher number of shoots per explant up to 3.8 shoots. The results were improved by culturing the one-fourth piece of 01.092 capitulums on MS medium fortified by 0.2 mg/l BAP and 0.02 mg/l NAA producing the highest shoot formation up to 8.5 shoots per explant with 28.7 leaves per explant and 2.1 cm leaf length. High multiple shoots were determined in third to fourth subculture periods and reduced thereafter with high multiplication rate noted on 01.092 clone. Shoots were easily rooted on half-strength MS medium supplemented with 2 g/l activated charcoal. Plantlets were transferred to ex vitro condition with 96.4% survivability of 03.045 clone using Cycas rumphii bulk and cocopeat (1:1, v/v) under spraying 1 g/l Growmore (32N:10P:10K) solution once week periodically. The route has high potential applied in qualified plantlet production for other Gerbera’s due to high shoots produced up to 35 shoots per whole young capitulum used.
<p>Produksi krisan di Indonesia memperlihatkan kecenderungan yang meningkat dalam 5 tahun terakhir. Selain sebagai bunga potong, krisan juga telah diminati konsumen sebagai tanaman hias pot. Namun, varietas krisan pot yang banyak beredar di pasaran saat ini merupakan varietas introduksi. Upaya perakitan varietas krisan pot dalam negeri telah dilakukan di Balai Penelitian Tanaman Hias dan telah diperoleh sejumlah genotip F1. Tujuan penelitian adalah untuk mengetahui beberapa parameter genetik dari genotip F1 krisan. Penelitian dilakukan di Kebun Percobaan Cipanas Balai Penelitian Tanaman Hias dari bulan September 2013 – Februari 2014. Penelitian menggunakan rancangan acak kelompok (RAK) dengan 96 genotip F1 krisan dan lima genotip tetua krisan pot yaitu Garden Mum Red, Surf, Sunny Ursula, Bonny, dan Miramar. Hasil penelitian pada populasi keseluruhan genotip menunjukkan nilai variabilitas genetik dan fenotipik yang luas untuk semua karakter pengamatan dengan nilai heritabilitas kategori tinggi pada 13 karakter dan sedang pada empat karakter. Pada masing-masing populasi, variabilitas genetik dan fenotipik yang luas dengan nilai heritabilitas tinggi terdapat pada karakter-karakter selain diameter batang, panjang daun, jumlah internode, panjang internode, jumlah cabang, dan lama kesegaran bunga untuk populasi A, diameter batang, jumlah internode, panjang internode, jumlah bunga per tanaman, jumlah cabang, dan lama kesegaran bunga untuk populasi B, diameter batang, panjang daun, lebar daun, jumlah internode, jumlah cabang, dan lama kesegaran bunga untuk populasi D, diameter tajuk dan lama kesegaran bunga untuk populasi E. Dengan demikian, seleksi untuk memperoleh genotip unggul akan efektif dilakukan pada selain karakter-karakter tersebut. Populasi C menunjukkan nilai variabilitas genetik yang sempit untuk semua karakter pengamatan. Setiap hasil persilangan krisan menghasilkan genotip-genotip F1 dengan warna bunga yang beragam serta diperoleh warna-warna bunga yang berbeda dari tetuanya.</p>
Potted chrysanthemums as one of high-demand potted ornamental plants are mostly cultivated using inorganic fertilizers. The organic fertilizers use as an alternative and complementary to inorganic fertilizers on potted chrysanthemum cultivation needs to be studied further. This study aims to determine the response of inorganic and organic fertilizer to agronomic characters of three potted Chrysanthemum varieties. The research was carried out in West Sumatra AIAT’s greenhouse from October to December 2020 using split-plot design with three replications. The main plots were fertilizer treatments (control, inorganic fertilizers, Bio-urine organic fertilizers) and sub-plots consisted of three potted chrysanthemum varieties (Armita, Avanthe, and Naura). Bio-urine organic fertilizers nutrient content N, P and K. The results showed that agronomic character of plant height, leaf length, leaf width, and the number of internodes resulted from type of fertilization treatments were significantly different. The highest plant height, leaf width, and number of internodes were significantly achieved in inorganic fertilizers then followed by Bio-urine organic fertilizers and control. The highest growth for the characters of plant height, stem diameter, leaf length, leaf width, petiole length, and flower diameter were attained from Avanthe. The interaction between fertilizers types and varieties was not significantly different in all observed characters.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.