plasmid (designated as pZM2,n) corresponding to plasmid pRUT883 of Stokes et al.12)) to pACYC184, and showed 2~3 times as high a copy numberas that of the shuttle vectors of Tonomuraet al.,10) in Z. mobilis. E. coli was grown in L-broth medium composed of 1% tryptone, 0.5% yeast extract and 1% NaCl, and Z. mobilis in RM medium3) composed of 1% yeast extract (Oxoid), 2% glucose and 0.2% KH2PO2, Plasmid DNA was isolated from E. coli or Z. mobilis by the procedure described by Birnboim and D6ly,13) and purified by phenol/chloroform extraction and ethanol precipitation. Restriction enzymes and T4 DNAligase were purchased from Boehringer Mannheim, New England Bio-Labs, or Takara Shuzo Co., Ltd., and reactions were carried out under the conditions recommended by the suppliers. Digested DNAs were analysed by agarose gel electrophoresis. Restriction enzyme analysis was carried out on plasmid pZM2isolated from Z. mobilis ATCC10988. The restriction map is shown in Fig. 1A. pZM2 had no sites for
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.