Purification and properties of an enzyme, zymolyase, which lyses viable yeast cells

Autolytic Release of Mannoproteins from Cell Walls of Saccharomyces cerevisiae

“…Recently, we have studied the mannoprotein species solubilized by purified Zymolyase (a pglucanase complex of bacterial origin; Kitamura et al, 1974) from S. cerevisiae walls . Essentially, material of high molecular mass and a 29 kDal mannoprotein were released ; these components appeared to interact preferentially with the glucan network of the wall (Shibata et al, 1983;Pastor et al, 1984).…”

Impact of mitochondrial activity on the cell wall composition and on the resistance to tannic acid in Saccharomyces cerevisiae.

“…Determination of zymolyase sensitivity (De Nobel et al, 1990;Kitamura et al, 1974). The cells were grown to an early exponential phase (2ϫ10 7 cells ml…”

Produção, purificação, clonagem e aplicação de enzimas líticas

“…Kitamura e Yamamoto 38 relataram que o microrganismo Arthrobacter luteus, isolado de resíduos de cervejarias, cresceu em meio contendo células de levedura ou β-1,3 glucana e produziu enzimas com atividade de lise de leveduras. O conjunto destas enzimas foi denominado zymolyase e requeria polímeros lineares de glicose com ligações β-1,3 como substrato específico.…”