Background: In India, Leukemia continues to be the largest contributor to cancer related mortality in children. Once the diagnosis of leukemia is suspected, a rapid evaluation and initiation of appropriate treatment is necessary. In addition biochemical test such as serum lactate dehydrogenase (LDH) activity and uric acid concentration have side by side gained importance in monitoring the prognosis of leukemia, especially during the phase of treatment. AIM: To evaluate serum lactate dehydrogenase and serum uric acid levels in patients suffering from leukemias. Material and Methods: The study was based on fifty patients of leukemia-acute or chronic, lymphoid or myeloid, diagnosed as a case of leukemia, both male and female, irrespective of their age attending out-patient Department or admitted in various wards of Government Medical College and Hospital, Amritsar were taken and their serum LDH and serum uric acid levels were estimated. Informed consent of the patient was taken. All newly diagnosed leukemia cases irrespective of their age and sex with no previous history of taking any chemotherapeutic drugs were included in the study. Quantitative estimation of serum lactate dehydrogenase is estimated using LDH (P-L) kit with normal reference values Serum of 230-460 U/L at 37 °C and quantitative estimation of serum uric acid was carried out using in vitro diagnostic kit (IVD) and enzymatic calorimetric method (Trinder) in clinical chemistry analyzers. Results: Mean serum uric acid and Mean LDH activity in study population was found to be 7.69±1.02 mg/dl and 794.07±227.62 IU/L respectively. Maximum rise in serum uric acid concentration was observed in AML type while Maximum rise in serum uric acid concentration was observed in ALL type. Both mean serum LDH concentrations and mean serum uric acid concentrations with TLC Levels in leukemic patients, we observed a strong correlation between increased TLC levels. On correlation analysis association between it was observed that the relation between serum uric acid and serum LDH would be considered statistically significant.
Conclusion:We can conclude that patients presenting with raised TLC along with hypercellular bone marrow and presenting with increased serum uric acid and LDH concentrations usually show poor prognosis in comparison to other leukemic patients.Thus continuous biochemical monitoring of serum LDH activity and uric acid concentration can play an important role in monitoring the prognostic aspect of the disease. Both serum uric acid and LDH measurement are easily available in laboratory and much cheaper parameters to assess disease progression.
This experiment was conducted to determine the fasting effects on anthropometric parameters and salivary antioxidant properties in girls and boys. Saliva samples were collected from eighteen healthy girls (control-09 & fasting-09 girls) and twelve healthy boys (control-06 & fasting-06 boys). Saliva was collected four times from all the subjects at a time interval of two hours. For anthropometric parameters, body weight, body temperature, pulse rate (PR), systolic blood pressure (SBP), diastolic blood pressure (DBP) was measured during each saliva collection time. Antioxidant parameters of all the saliva samples were evaluated. Most of the anthropometric parameters like temperature, PR, SBP, and DBP of the fasting group was significantly (p<0.5) decreases than the control group of both boy's and girl's in the different time interval. Antioxidant properties were significantly (p<0.5) higher in the fasting group of girls, but the insignificantly different level was found in the boy's group. This study was indicating that fasting affected the anthropometric parameters (PR, SBP, and DBP), mainly in the girls. Salivary antioxidant properties were mostly lower in the girls' fasting group. Therefore, saliva may be a useful stress biomarker during fasting conditions.
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