Plantago asiatica mosaic virus (PlAMV) is a member of the genus Potexvirus and has an exceptionally wide host range. It causes severe damage to lilies. Here we report on the complete nucleotide sequences of two new Japanese PlAMV isolates, one from the eudicot weed Viola grypoceras (PlAMV-Vi), and the other from the eudicot shrub Nandina domestica Thunb. (PlAMV-NJ). Their genomes contain five open reading frames (ORFs), which is characteristic of potexviruses. Surprisingly, the isolates showed only 76.0-78.0 % sequence identity with each other and with other PlAMV isolates, including isolates from Japanese lily and American nandina. Amino acid alignments of the replicase coding region encoded by ORF1 showed that the regions between the methyltransferase and helicase domains were less conserved than other regions, with several insertions and/or deletions. Phylogenetic analyses of the full-length nucleotide sequences revealed a moderate correlation between phylogenetic clustering and the original host plants of the PlAMV isolates. This study revealed the presence of two highly divergent PlAMV isolates in Japan.
In this paper, some results of crack propagation analyses of deep initially semi-elliptical flaws under assumed residual stress fields are presented. The crack propagation analyses were performed by using a software system that has been developed by Okada and his colleagues. It is based on a conventional finite element program but uses the quadratic tetrahedral finite elements to model the structure with the crack. The finite element model with the crack can be generated in an automated manner. The stress-intensity factor computations are performed by using the virtual crack closure-integral method (VCCM) for the quadratic tetrahedral finite element which was also proposed by Okada and his colleagues. The automatic meshing scheme for the crack propagation analyses has also been developed by the authors.
By the authors’ previous publication, it was shown that the stress intensity factor of deep semi-elliptical surface flaw under assumed residual stress field reached its maximum value at the mid-depth of the crack. Hence, in present study, in order to investigate the feature of the crack propagation of deep surface cracks, we are conducting crack propagation analyses that can predict the crack extension from each point along the crack front for an arbitrary shaped surface flaw. It can also account for material anisotropy in the crack propagation behavior. Then, the SCC crack propagation analyses for a deep semi-elliptical surface flaw in a plate under assumed residual stress fields are being conducted.
The results of the crack propagation analyses suggest that the shapes of the crack after the SCC crack propagation may not be exact semi-elliptic in its shape. In this paper, the analytical procedures and some results are presented. The same analytical procedures can be adopted to perform fatigue crack propagation analyses.
Engineering2641Yamazaki , Nodeq Chiba , 278 − 8510Japa 皿 粫 en crack analyses are perfc ) rmed f ( )r the structural integrity eva 】 uations , 廿1e hexahedral elements are adopted in generally . It is very time consuming to bui ] d the finite element mesh using the hexahedral elements . Therefbre , automated mesh generation by using the tetrahedral elements is adopted in present softwa 爬 system . AutOmated parametric analysis system f} om the meshing to the stress intensity factor compulation can eliminate the manual operations completely . In this paper, automated parametric system is briefly described and a numerica1 example is presented . Keッ Words:Finite Element Method , Stress Intensity Factors
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