To improve liposomes’ usage as drug delivery vehicles, cryoprotectants can be utilized to prevent constituent leakage and liposome instability. Cryoprotective agents (CPAs) or cryoprotectants can protect liposomes from the mechanical stress of ice by vitrifying at a specific temperature, which forms a glassy matrix. The majority of studies on cryoprotectants demonstrate that as the concentration of the cryoprotectant is increased, the liposomal stability improves, resulting in decreased aggregation. The effectiveness of CPAs in maintaining liposome stability in the aqueous state essentially depends on a complex interaction between protectants and bilayer composition. Furthermore, different types of CPAs have distinct effective mechanisms of action; therefore, the combination of several cryoprotectants may be beneficial and novel attributed to the synergistic actions of the CPAs. In this review, we discuss the use of liposomes as drug delivery vehicles, phospholipid–CPA interactions, their thermotropic behavior during freezing, types of CPA and their mechanism for preventing leakage of drugs from liposomes.
Introduction: Numerous locally accessible plants in Nepal are left unseen. So, exploring their antioxidant activity for medicinal purposes can be beneficial in treating various diseases. Antioxidants have great importance in terms of reducing oxidative stress that causes damage to biological molecules. The qualitative analysis of chemical constituents using a chromatographic technique like TLC plays a pivotal role in this aspect. The present study aimed at performing chemical profiling using thin-layer chromatographic technique and evaluation of the antioxidant activity of selected medicinal plants.
Methods: Firstly, TLC profiling of 70% methanolic extracts of eighteen medicinal plants was done using preparative TLC plate in two different ratio of chloroform, methanol and water. Then, antioxidant activity was tested by DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical scavenging assay by using a 96 well plate method at wavelength 510 nm in which Trolox was taken as standard.
Results: Flavonoids, tannins, saccharides, and phenols were identified from TLC profiling. The yield value of Sapium insigne leaves extracts i.e. 20.52% was highest and Monochoria vaginalis leaves possess the least i.e. 3.93%. Solena heterophylla leaves extract with IC50 amount i.e. 21 µg/ml was found most potent among all the plant extracts.
Conclusion: The results imply that the extract of ethnomedicinal plants is rich with a variety of phytochemicals, which can be used as natural antioxidants. However, further studies are warranted to isolate and identify the chemical and biological properties of obtained extracts for the provision of scientific evidence for traditional uses.
Nanomedicine is a branch of medicine using nanotechnology to prevent and treat diseases. Nanotechnology represents one of the most effective approaches in elevating a drug‘s treatment efficacy and reducing toxicity by improving drug solubility, altering biodistribution, and controlling the release. The development of nanotechnology and materials has brought a profound revolution to medicine, significantly affecting the treatment of various major diseases such as cancer, injection, and cardiovascular diseases. Nanomedicine has experienced explosive growth in the past few years. Although the clinical transition of nanomedicine is not very satisfactory, traditional drugs still occupy a dominant position in formulation development, but increasingly active drugs have adopted nanoscale forms to limit side effects and improve efficacy. The review summarized the approved nanomedicine, its indications, and the properties of commonly used nanocarriers and nanotechnology.
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