Introduction: Numerous locally accessible plants in Nepal are left unseen. So, exploring their antioxidant activity for medicinal purposes can be beneficial in treating various diseases. Antioxidants have great importance in terms of reducing oxidative stress that causes damage to biological molecules. The qualitative analysis of chemical constituents using a chromatographic technique like TLC plays a pivotal role in this aspect. The present study aimed at performing chemical profiling using thin-layer chromatographic technique and evaluation of the antioxidant activity of selected medicinal plants. Methods: Firstly, TLC profiling of 70% methanolic extracts of eighteen medicinal plants was done using preparative TLC plate in two different ratio of chloroform, methanol and water. Then, antioxidant activity was tested by DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical scavenging assay by using a 96 well plate method at wavelength 510 nm in which Trolox was taken as standard. Results: Flavonoids, tannins, saccharides, and phenols were identified from TLC profiling. The yield value of Sapium insigne leaves extracts i.e. 20.52% was highest and Monochoria vaginalis leaves possess the least i.e. 3.93%. Solena heterophylla leaves extract with IC50 amount i.e. 21 µg/ml was found most potent among all the plant extracts. Conclusion: The results imply that the extract of ethnomedicinal plants is rich with a variety of phytochemicals, which can be used as natural antioxidants. However, further studies are warranted to isolate and identify the chemical and biological properties of obtained extracts for the provision of scientific evidence for traditional uses.
<p class="abstract"><strong>Background:</strong> Urinary tract infection (UTI) is the most common infection caused by the presence and growth of pathogenic microorganisms within the human urinary system into the lower and upper genito-urinary tract. UTI continues to be major health problems in developing countries like Nepal.</p><p class="abstract"><strong>Methods:</strong> A prospective observational study was conducted from June 2019 to November 2019 in the outpatient and inpatient department of Manipal teaching hospital. The face-to-face interview was adopted in 137 UTI patients with the census sampling technique. </p><p class="abstract"><strong>Results:</strong> A total of 137 patients (121 out-patients and 16 in-patients), 82 (59.85%) female, and 55 (40.15%) males were enrolled in which UTI was observed in more than half of the sample in the aged group of 25-54. <em>Escherichia coli</em> was the most common uropathogens isolated whereas dysuria was the most common presenting symptom among the patients. Cefixime 200 mg followed by nitrofurantoin 100 mg was the most commonly prescribed medicine for UTI. Disodium hydrogen 1.40 g oral liquid, pantoprazole 40 mg tablet, and multivitamins were commonly prescribed supportive medications. The empirically prescribed average cost was Nepalese rupee (NRs.) 820.44 whereas the total definitive average cost was NRs. 305.54. The average cost of medical management for in-patients was NRs. 2316.55 including registration, urine analysis, investigation bed charge, and other consumable charges as well as discharge medicine cost.</p><p class="abstract"><strong>Conclusions:</strong> With lower cost, higher sensitivity, and high prescription, cefixime, and nitrofurantoin could be better drugs for the management of UTI among outpatient department (OPD) patients while ceftriaxone could be better for in-patients.</p><p> </p>
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