Most important during probiotic selection are gastric acid and bile tolerance, the adhesion to the luminal epithelium to colonize the lower gastrointestinal tract of a human and safety for human consumption. The aim of this study was to evaluate the selected probiotic in vitro properties of Lactobacillus spp. Strains isolated from traditional fermented food. A total 38 strains were isolated from the pickled samples and 14 were identified as Lactobacillus spp. The survival of almost all strains after incubation at pH 2.5 did not change markedly, and remained at above 90 % (10(9) CFU/mL). The strains also exhibited a high survival rate at pH 3.5 (>90 %), whereas pH 1.5 all were died. Just four strains could survive 90 min. at pH 1.5 (<39 %). The incubation with 0.2 % bile salt solution resulted in a survival rates of 81-94 % after 24 h, whereas after incubation in 2 and 4 % bile salt solution it was 59-94 %. All tested strains showed very good and good resistance to 0.4 % phenol addition, however only Lb. johnsonii K4 was able to multiply. The hydrophobic nature of the cell surface of the tested strains was moderated recording hydrophobicity of Lb. johnsonii K4 and Lb. rhamnosus K3 above 60 %. Safety evaluation excluded four of tested strains as candidate probiotics, according to antibiotic resistance patterns and certain metabolic activities. On the basis on the results 10 of the selected Lactobacillus strains are safe and can survive under gastrointestinal conditions, which requires them to future in vitro and in vivo probiotic studies.

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Objectives: The aim of the study was to evaluate the safety and effectiveness of cosmetic products containing two derivatives of vitamin K on skin with vascular problems. Materials and methods: The study was conducted in two groups, 24 people, and the other included 22 participants, mean age 50. The participants applied creams twice a day for a period of 4 weeks. Creams were applied on the right half of the face-concentration of 0.5% and 0.1%-on the left. The participants completed a questionnaire and a measurement was performed using standardized equipment Multi Probe Adapter (MPA) Courage-Khazaka and VISIA Canfield. Results: Application creams with MQ and Epoxy MQ derivatives at a concentration of 0.1% and 0.5% had the effect of reducing redness of the skin at a comparable level. All of formulations did not contribute to decrease melanin level. The hydration was increased after application MQ (0.1% and 0.5%) adequately 14% and 13% in about 60% participants. The elasticity was significantly improved in all groups. Conclusion: Derivatives of vitamin K such as Epoxy MQ and MQ used in 0.1% and 0.5% give comparable results reducing redness of the skin. The improvement of erythema was more significant with 0.1% concentration (MQ and Epoxy MQ).