Objective: This study aims to prove the effect of supplementation of bitter melon leaves extract on glycemic status in diabetic foot ulcer (DFU). Materials and Methods: This study used a randomized, double-blinded, and placebo-controlled trial. Thirty DFU patients based on PEDIS 1-8 scores who met the criteria were divided into 2 groups consisting of 15 patients as a treatment group with the administration of bitter melon leaves extract at a dose of 6 g/day and 15 patients as a control group of with placebo. This intervention was conducted in 4 weeks. Glycemic status was measured by the value of glycated albumin and examined at baseline and at the end of treatment. Data were analyzed using paired t-test and independent t-test. Results: After 4 weeks of treatment, there was an increase in the baseline value of glycated albumin in the treatment group from 24.5 ± 11.7% to 25.8 ± 12.7% or an increase of 1.3 ± 6.3% and a decrease in the control group from 27.5 ± 13.1% to 25.8 ± 8.5% or a decrease of 1.7 ± 12.4%. The results of the analysis of the effect of supplementation of bitter melon leaves extract on the value of glycated albumin were not significant (p = 0.99). Conclusion: The supplementation of bitter melon leaf extract with a dose of 6 g / day did not significantly affect the value of glycated albumin. Bangladesh Journal of Medical Science Vol.20(2) 2021 p.281-287

ABSTRACT. The corticotrophin-releasing hormone (CRH) and proopiomelanocortin (POMC) genes are considered to play an important role in the growth and development of mammals. In this study, the bovine CRH and POMC genes were characterized to detect genetic variation at these loci in relation to economic traits in Korean cattle (Hanwoo). Nine single nucleotide polymorphisms (SNPs; C148T, A186G, A234C, G269A, G1030A, G1084A, A1136C, G1179C, and A1439G) were detected in the CRH gene, and six SNPs (C7017T, A7027T, C7050T, G7063T, C7160T, and C7221T) were detected in the POMC gene. Three SNPs in the CRH gene (G1030A, G1084A, and G1179C) were missense mutations, and three SNPs in the POMC gene (C7017T, A7027T, and C7160T) were missense mutations. Statistical analysis indicated that one CRH polymorphism (G1084A) was significantly (P = 0.05) associated with the longissimus dorsi muscle area (LMA), and a POMC polymorphism (C7221T) significantly influenced LMA and marbling scores. A significant interaction was detected between CRH and POMC in relation to carcass weight and LMA. These results indicate that CRH and POMC may be candidate genes for carcass traits, and suggest that the interaction between CRH and POMC strongly affects carcass traits in cattle.

The purpose of this study is to determine the effect of oral Bitter Melon (Momordica charantia L.) leaves extract on the serum VEGF level in diabetic foot ulcers. The trial was randomized, double-blinded, and placebo-controlled. Thirty diabetic foot ulcer (DFU) patients with a PEDIS score of 1-8 who met the criteria were divided into two groups: The treatment group received the bitter melon leaves extract orally at a dose of 6 g/day, while the control group received a placebo. The intervention lasted four weeks, and baseline and end-of-treatment VEGF serum levels were determined. The paired t-test and independent t-test were used to analyze the data. After four weeks of treatment, the baseline VEGF serum level in the treatment group decreases from 431.5248.5 to 236.9158 pg/ml (the decline begins at 194.5197.9 pg/ml). Meanwhile, in the control group, the concentration decreases from 382.4351,4 to 191.6121.9 pg/ml (the concentration decreases to 190.8324.9 pg/ml). The effect of oral supplementation with Bitter Melon leaves extract on VEFG serum levels was found to be insignificant (p=0.39). Oral bitter melon leaf extract supplementation was not shown to increase VEFG serum levels in DFU patients.