(2), 95-107 Effects of etoposide (VP-16) and cytosine arabinoside (Ara-C) on the cell cycle of HL-60 and THP-1 cells were studied by flow cytometry using the bromodeoxyuridine (BrdU)/DNA assay technique to investigate the efficacy of VP-16 for monocytic leukemia cells. VP-16 inhibited the proliferation of THP-1 cells more strongly than that of HL-60 cells at any concentrations used at 24 and 48 hr. VP-16 arrested HL-60 and THP-1 cells in the G2 /M phase and reduced them in the Go/G1 and early S phase at higher concentrations. There was no significant difference in the percentage of G2/M phase cells at the same concentration between both cells. However, reduction in the Go/G, and early S phase cells was more marked in THP-1 than HL-60 cells significantly. On the other hand, Ara-C perturbed the cell cycle of HL-60 cells more than that of THP-1 cells at 24 and 48 hr. These results suggest that the effects of VP-16 on the cell cycle may be more intense in THP-1 than HL-60 cells, and support the efficacy of VP-16 for treating monocytic leukemia in vivo. flow cytometry; cell cycle; bromodeoxyuridine (BrdU); etoposide (VP-16); cytosine arabinoside (Ara-C) Etoposide (VP-16), a newly developed semisynthetic derivative of podophyllotoxin (Loike and Horwitz 1976;Roberts et al. 1980), is recently available for the treatment of acute leukemias and malignant lymphomas, and has been found useful for relapsed or refractory cases (Mathe et al. 1974;Bishop et al. 1990). This drug is considered more effective for patients with monocytic leukemia than for those with other types of leukemia (Mathe et al. 1974;Bernasconi et al. 1982;
Ordered historically, I shall update my earlier conviction that a consistent interpretation of all the non-terrestrial gamma-ray bursts can be obtained in terms of nearby Galactic neutron stars, at distances d within 10 < ∼ d/pc < ∼ 500.The γ-ray bursts made their scientific appearance at the 1974 Texas Symposium on Relativistic Astrophysics when they had just been declassified by American militaries, and were introduced to the community by Malvin Ruderman. In his superb presentation, Ruderman (1975) offered more proposed classes of sources than detected bursts, viz.
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