Tobacco (Nicotiana tabacum L.) is an important agricultural crop plant for the economy of many countries. Assessment of the genetic diversity of cultivated tobacco varieties is of importance for long-term tobacco improvement. Microsatellite markers are currently the marker system of choice for genetic analysis of allopolyploid plants. In this study, we evaluated the use of 30 microsatellite markers for identification of 10 varieties of cultivated tobacco in the Republic of Macedonia. We found 24 of the microsatellite markers to be polymorphic and sufficient for identification of these varieties. Cluster analysis showed that Macedonian tobacco varieties are classifiable into three distinct groups.
SUMMARYInvestigations were made with the new burley variety B 2/93, bred in the Tobacco Institute Prilep, on alluvial soil type in the producing region of Ohrid and Struga. Four rates of nitrogen fertilizer (55, 100, 150, and 200 kg ha 1 ) and a constant rate of phosphorus and potassium were applied in the investigation. Based on the results obtained, it can be stated that fertilization and irrigation have a significant influence on the anatomic structure of burley tobacco leaf. The best developed anatomic structure, with harmonically increased palisade and spongy parenchyma, was found in the variant fertilized with 150 kg ha -1 N. The cells of the parenchyma are not dense, and the spongy cells are few and irregularly distributed, forming large intercellular spaces between them. As a result of the interactive effect of irrigation and fertilization, this treatment is distinguished by a thick and soft leaf lamina with excellent adsorptive power, high filling capacity and, consequently, better quality. [Beitr. Tabakforsch. Int. 21 (2005) 345-349]
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