Gladiolus is one of the most potential bulbous cut flower crops cultivated widely in India due to its attractive and long lasting spikes. The plant is propagated vegetatively through corms and cormels, but due to low rate of multiplication, its cultivation is hindered. Also, it is grown through underground modified stems, they are more often attacked by soil borne diseases such as Fusarium corm rot. In-vitro propagation techniques, assumes significance, especially for securing rapid multiplication of quality planting propagules using dormant cormlet explants and media. The results of the study revealed that the MS growing medium supplemented with 2 mg/l BAP showed improved results in respect of sprouting of explants (54.46 %), days required for sprouting of explants (8.68 DAI) and shoot length at 30 (6.32 cm), 60 (12.40 cm) and 90 (13.21 cm) DAI. The in vitro regenerated gladiolus plantlets when placed in the rooting MS medium supplemented with 4 mg/l IBA showed earlier root initiation (8.31 DAI) and higher number of roots/shoot (5.37 DAI) as compared to the rest of the treatment combinations. Hardening medium consisting of garden soil + sand + vermicompost (1:1:1) showed greater survivability (50.98 %) of plantlets. Thus, the study has been initiated an efficient protocol for in-vitro propagation of gladiolus through cormlets cultured in MS media containing BAP and IBA as shooting and rooting plant growth regulators and subsequent acclimatization in garden soil + sand+ vermicompost medium.
An experiment was conducted to determine the combined effect of biofertilizers and biocontrol agents on growth and yield of brinjal (Solanum melongena L.) under low cost polyhouse during off season in the experimental polyhouse of the College of Agricultural Engineering and Post Harvest Technology (CAEPHT), Central Agricultural University (CAU), Ranipool, Sikkim, India. The experiment was planned with 3 treatments and six replications viz. T1- Farm Yard Manure 5 kg/m2, T2- FYM 5 kg/m2 + biofertilizer (a mixture of Azotobactor + PSB at the rate 10 g/kg FYM each), T3- FYM 5 kg/m2 + biofertilizer + biocontrol agent (a mixture of Pseudomonas fluorescens + Trichoderma at the rate 5 g/kg FYM each). There was a significant variation in vegetative growth and yield among all the treatments. The maximum plant height (45.62 cm), the number of branches/ plant (11.17) and the number of leaves/ plant (50.05),the number of fruits/ plant (38.9) and fruit yield/ plant (810 g) were observed with treatment T3 which was at par with the treatment T2 and were significantly higher than the treatment T1 receiving FYM singly. Organic manure (FYM) inoculated with biofertilizers may therefore, be recommended for organic brinjal production for cultivation under low cost polyhouses in Sikkim (India) and application of biocontrol agents may be limited to areas having some history of occurrence of diseases because it involves an extra production cost in the application of biocontrol agents.
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