Three binary dye mixtures prepared from the basic textile dyes Astrazon Blue FGRL (AB), Astrazon Red GTLN (AR), and Astrazon Golden Yellow GL-E (AY), were employed to demonstrate two different mixture characteristics. The first type of mixture (AB + AY) was the case where the optical absorbance spectra of the dye mixtures exhibited double peaks. Each peak occurred at the same wavelength as the single dye and the area under each light absorbance curve varied with the concentration of the dye in the solution. The second type was the case of the other two mixture combinations (AR + AY and AB + AR) whose spectra exhibited a single peak. In this case, shifts of the spectral peaks were observed as the composition of the mixture changed, and the area beneath the light absorbance curve corresponded to the quantity of the dyes in the mixture. With these techniques, it was possible to determine the quantity of each individual dye component in the binary dye mixture. The application of these techniques was demonstrated through estimation of the amount of dye adsorbed from simulated dye wastewater by dried biomass of Caulerpa lentillifera.
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