ABSTRACT. Penelitian ini bertujuan mengevaluasi keberhasilan IB menggunakan metode dosis tunggal dan ganda pada sapi Madura. Penelitian menggunakan 25 ekor dengan metode IB dosis tunggal dan 25 ekor dengan metode IB dosis ganda. IB menggunakan semen beku sapi Madura yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari, Malang. Teknik deposisi semen adalah 4+ (deep insemination) yaitu pada posisi cornua uteri selanjutnya dilakukan IB dosis tunggal (jam ke-8 setelah menunjukkan tanda-tanda berahi) dan IB dosis ganda (jam ke-2 dan ke-8 setelah menunjukkan tanda-tanda berahi). Akseptor diinjeksi dengan Bio ATP+ merek “Rheinbio” 10 ml per ekor dan pemberian pakan konsentrat merek “Novo NC62” 1 kg per ekor per hari selama tujuh hari setelah IB. Variabel penelitian meliputi Non Return Rate (NRR), Conception Rate (CR), dan Service Per Conception (S/C). Hasil penelitian menunjukkan bahwa IB dosis tunggal menghasilkan nilai NRR 1 sebesar 92%, NRR 2 sebesar 80%, CR sebesar 60%, dan S/C sebesar 1,71. Sedangkan pada IB dosis ganda menghasilkan nilai NRR 1 sebesar 92%, NRR 2 sebesar 84%, CR sebesar 68%, dan S/C sebesar 1,53. Kesimpulan penelitian adalah nilai NRR, CR, dan S/C pada sapi Madura dengan IB dosis ganda lebih baik daripada dengan IB dosis tunggal, dan IB dosis ganda dapat meningkatkan conception rate pada sapi Madura. (The success different of artificial insemination using single and double dosage method of Madura cows) ABSTRAK. This study aims to evaluate the success of AI using methods single and double dosage plus in Madura cows. The study used 25 Madura cow using the AI method single dosage and 25 Madura cow using the AI method double dosage. AI uses frozen semen from Madura cows produced by the Singosari Center for Artificial Insemination. The semen deposition technique is 4+ (deep insemination) in the cornua uteri position then AI implemented with a single dosage (8th hours after showing signs of estrus) and double dosage (2nd and 8th hours after showing signs of estrus). The acceptors were injected with 10 ml of "Rheinbio'' brand Bio ATP+ per cow and 1 kg of concentrate "Novo NC62" brand per cow per day was given for seven days after IB. Research variables include Non Return Rate (NRR), Conception Rate (CR), and Service Per Conception (S/C). The results showed that AI of single dosage had value NRR 1 of 92%, NRR 2 of 80%, CR of 60%, and S/C of 1.71. While AI of double dosage had value NRR 1 of 92%, NRR 2 of 84%, CR of 68%, and S/C of 1.53. In conclusion, the value of NRR, CR, and S/C in Madura cows with AI double dosage was better than AI single dosage, and AI double dosage could increase the conception rate in Madura cows.
One of the potencies of Madura cow genetics is its adaptation ability to the tropical climate. Yet in most cases, the reproduction efficiency of the Madura cows has not reached the optimum point. This research examines the use of double-dose artificial insemination compared to a singledose on Madura cows. The study was conducted in Daramista, Lenteng District, Sumenep Regency, Madura, East Java. This research utilized an experimental field method with purposive sampling on 100 Madura cows, with a body condition score of 3-4, aged about 2-6 years old. The treatments were divided into two groups, T0 and T1. T0 cows received single-dose artificial insemination treatment on the 8 th h after estrus and T1 cows received double-dose artificial insemination treatment on the 2 nd and 8 th h after estrus. The Singosari National Artificial Insemination Centre produced Madura bull frozen semen straws used in this research. Before inseminating, frozen semen straws were thawed at 28°C for 30 sec. This research observed some variables, including; Non-Return Rate (NRR1, NRR2, NRR3), Service per Conception (S/C), Conception Rate (CR), and estrus quality condition as supporting data. The researchers analyzed the data descriptively and through chi-square analysis. The result of the study showed that NRR1, NRR2, and NRR3 of T0 were 94, 84, and 70%, while NRR1, NRR2, and NRR3 T1 were 92, 86, and 82%. The CR of T0 was 64% compared to the CR of T1 at 76%. The double dose insemination method at different times on the 2 nd and 8 th h after estrus can increase the reproduction performance in Madura cows.
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