Anthocyanins play an important role in physiological functions related to human health. The objective of this study was to investigate the profiles of anthocyanins in the immature purple pods and black seeds of yard-long beans ( Vigna unguiculata ssp. sesquipedalis L.) using high-performance liquid chromatography (HPLC) with diode array detection and electrospray ionization/mass spectrometry (DAD-ESI/MS) analysis. The individual anthocyanins were identified by comparing their mass spectrometric data and retention times. In the purple pods, five individual anthocyanins were identified: delphinidin-3-O-glucoside (2), cyanidin-3-O-sambubioside (4), cyanidin-3-O-glucoside (5), pelargonidin-3-O-glucoside (7), and peonidin-3-O-glucoside (8). From the black seed coat of the yard-long beans, seven anthocyanins were identified, including delphinidin-3-O-galactoside (1), cyanidin-3-O-galactoside (3), petunidin-3-O-glucoside (6), and malvidin-3-O-glucoside (9), together with compounds 2, 5, and 8. In this study, we report for the first time anthocyanin profiles for the pod and seed coat of yard-long beans.
This study assessed the altitudinal variations in the anthocyanin and isoflavone contents of six black seed coated soybean [Glycine max (L.) Merrill] cultivars. The black soybean cultivars Heugcheong, Seonheuk, Geomjeong 1, Geomjeong 2, Cheongja 2, and Cheongja 3 were planted at Milyang (12 m above mean sea level -low altitude) and Muju (600 m -high altitude), Korea on 10 June 2005 and 2006. The total anthocyanin and isoflavone contents and individual components were investigated by reverse-phase high performance liquid chromatography (HPLC). All black soybean cultivars cultivated in high altitude possessed significantly higher total anthocyanin (p < 0.01) and isoflavone (p < 0.01) contents than those grown in low altitude. For anthocyanin composition, cyanidin-3-O-glucoside, cyanidin-3-O-galactoside, and peonidin-3-O-glucoside contents were significantly higher while delphinidin-3-Oglucoside contents was significantly lower at high altitude. The composition of individual isoflavones, 6"-O-malonyldaidzin, and 6"-O-malonylgenistin contents significantly increased at high altitude.
A new soybean cultivar for soybean sprout, 'Wonheug' was developed at the National Institute of Crop Science (NICS) in 2009. 'Wonheug (Iksan63)' was released from the cross between F1 of YS1286 (Tawonkong/Jinju#1) and Jinju#1. It has purple flower, oval shaped leaf, tawny pubescence and small black seed (8.8 g/100 seed). Maturing date of 'Wonheug' is ten days later than check cultivar, 'Tawonkong'. Even though mosaic symptom can be observed by artificial inoculation, 'Wonheug' is tolerant to virus and bacterial pustule at field. It has similar tolerance to a check cultivar for excess water and seed spot. 'Wonheug' produce more soy-sprout than a check cultivar because it shows high in germination ratio, low in rate of abnormal germination and hard seed. The average yield of 'Wonheug' was 2.34 ton per hectare in the regional yield trial (RYT) carried out for three years from 2007 to 2009, which was 12 percent higher than check cultivar, 'Tawonkong'. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 서 언 콩나물은 시설재배가 발달되기 이전에, 일주일의 시간과 물만 있으면 계절과 관계없이 신선한 채소를 공급해 주던 식 품재료이다. 콩나물용으로는 주로 100~120 mg/seed의 소립 인 콩이 많이 이용되고 있는데, 이는 종자활력과 배축신장성 이 좋으며 콩나물의 수율이 높기 때문이다(Kwon et al. 1981, Lee et al. 1992). 현재 재배특성이 우수한 황색 종피인 품종들이 상업적으로 많이 이용되고 있다. 그러나, 가정에서 콩나물을 재배해서 이 용하던 시기에는 유색 종피를 가진 콩이 많이 이용되어 왔다. 혼합색 혹은 녹색콩이 황색콩에 비하여 저장기간의 경과에 따른 발아율의 감소가 적다고 한다(Hwang 1997). 또한, 유색 콩으로 재배한 콩나물이 황색 콩으로 재배한 콩나물에 비해 질감과 맛이 좋은 것으로 인식되고 있어 유색 콩나물용 콩에 대한 수요가 상존하고 있다. 2010년도 나물용 콩의 국내생산량은 1만 2,060톤, 수입량 4만 177톤으로 자급율은 30%에 머물고 있으며 대부분 중국 韓育誌(Korean J. Breed. Sci.) 45(3), 2013
This experiment was conducted to select suitable cultivars and evaluate growth characteristics to get basic information for sprouting peanut. On sprouting peanut, it showed a rapid increase in trans-resveratrol content that has effects on anti-cancer, anti-inflammatory, blood-sugarlowering and other beneficial cardiovascular in mouse. For this experiment, characteristics of peanut sprouts were tested in 37 cultivars grown for 7 days at 26℃ temperature. There were a lots of variations in the growth characteristics among cultivars as followers : The range of 100 grain weight was 56 to 142 g, hypocotyl length was 4.3 cm to 5.8 cm, diameter of hypocotyl was 5.0 to 8.0 mm, epicotyl length was 0.8 cm to 4.6 cm, seedling ratio per seed number was 84% to 100%, weight per seedling was 4.9 g to 8.4 g, the rate of hypocotyl cleavage was 0% to 46%, the content of trans-resveratrol was 22.5 μg/g to 88.2 μg/g and sprout yield was 360% to 820%. The selection points considered were high sprout yield, high seedling rate, high resveratrol content, low brownish cotyledon, no hypocotyl cleavage, and fat hypocotyl etc. The best cultivar selected was 'Jokwang' that showed 7.8 mm diameter, clean cotyledon color, 100% seedling rate, 0% hypocotyl cleavage, 63.3 μg/g resveratrol, and 820% sprouting yield. This cultivar was expected to be of use as a new food and nutraceutical material. Relationship between growth characteristics showed that root length had significant positive correlations with epicotyl length, resveratrol content and sprouting yield but negative correlations with hypocotyl diameter and cleavage. Hundred grain weight showed negative correlations with resveratrol content, seedling rate and sprouting yield but positively correlated with curved hypocotyl rate and hypocotyl cleavage positively. This result showed small grain seed will be more appropriate for sprouting peanut.
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