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The accumulation of transcripts for the major gluten storage proteins was assessed during grain development in wheat plants (Triticum aestivum L. ‘Butte 86’) grown under seven controlled environments where temperature, water, and fertilizer conditions were varied after anthesis. Transcripts within the α‐, γ‐ and ω‐gliadin, low molecular weight glutenin subunit (LMW‐GS), and high molecular weight glutenin subunit (HMW‐GS) gene families followed similar patterns of accumulation throughout grain development under each environmental regimen. Under moderate daytime temperatures in plants that were well watered and fertilized, transcripts from all gene families were detectable by eight days postanthesis (8 DPA), were present at high levels until ≈34 DPA, and disappeared between 36 and 38 DPA. Under high temperature regimens, transcripts from all gene families appeared slightly earlier, but the time frame of accumulation was shorter. The presence or absence of postanthesis fertilizer did not alter the temporal regulation of the gluten genes under the moderate temperature regimen. However, under the high daytime temperature regimen, transcripts disappeared slightly earlier in kernels from plants that did not receive postanthesis fertilizer. Reverse transcriptase polymerase chain reaction (RT‐PCR) using primer pairs for specific HMW‐GS and LMW‐GS transcripts revealed that individual genes within each family exhibited identical patterns of temporal regulation under different environmental conditions.

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A total of 37 original cDNA libraries and 9 derivative libraries enriched for rare sequences were produced from Chinese Spring wheat (Triticum aestivum L.), five other hexaploid wheat genotypes (Cheyenne, Brevor, TAM W101, BH1146, Butte 86), tetraploid durum wheat (T. turgidum L.), diploid wheat (T. monococcum L.), and two other diploid members of the grass tribe Triticeae (Aegilops speltoides Tausch and Secale cereale L.). The emphasis in the choice of plant materials for library construction was reproductive development subjected to environmental factors that ultimately affect grain quality and yield, but roots and other tissues were also included. Partial cDNA expressed sequence tags (ESTs) were examined by various measures to assess the quality of these libraries. All ESTs were processed to remove cloning system sequences and contaminants and then assembled using CAP3. Following these processing steps, this assembly yielded 101,107 sequences derived from 89,043 clones, which defined 16,740 contigs and 33,213 singletons, a total of 49,953 "unigenes." Analysis of the distribution of these unigenes among the libraries led to the conclusion that the enrichment methods were effective in reducing the most abundant unigenes and to the observation that the most diverse libraries were from tissues exposed to environmental stresses including heat, drought, salinity, or low temperature. G ENOME projects are progressing at an unprecehigh-throughput technologies (http:/ /www.ncbi.nih.gov/ Genomes/index.html). However, among higher plants dented pace for a wide range of species from bacterial to human due to the continuing improvement of only the two model species, Arabidopsis (Arabidopsis thal-1

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