Genes encoding the PR-4 protein wheatwin are developmentally regulated in wheat grains and respond to high temperatures during grainfill

Altenbach, Susan B.; Kothari, Kerry M.; Tanaka, Charlene K.; Hurkman, William J.

doi:10.1016/j.plantsci.2007.04.007

Cited by 26 publications

(18 citation statements)

References 23 publications

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“…Whatever the cultivar studied, high temperatures have been shown to shorten the effective grain fi lling period, and may signifi cantly increase protein concentration because of more dramatic effect on starch than on protein accumulation Blumenthal et al 1991aBlumenthal et al , b , 1995Corbellini et al 1998 ;Triboï et al 2003 ). Many studies have addressed the responses of wheat grain to high temperatures during grain fi lling using either transcriptomics tools (Altenbach and Kothari 2004 ;Altenbach et al 2007 ;Hurkman et al 2003 ;Perrotta et al 1998 ) or proteomics approaches (Hurkman et al 2009 ;Laino et al 2010 ;Majoul et al 2003Majoul et al , 2004Majoul-Haddad et al 2013 ;Skylas et al 2002 ;Vensel et al 2005 ). In the present study, major events occurring in the wheat endosperm in response to high temperatures during grain fi lling were investigated using proteomics approaches.…”

Section: High Temperature Is a Major Factor Affecting Wheat Productionmentioning

confidence: 99%

Coping with Wheat Quality in a Changing Environment: Proteomics Evidence for Stress Caused by Environmental Changes

Branlard

Lesage

Bancel

et al. 2015

Advances in Wheat Genetics: From Genome to Field

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High temperatures (HTs) during grain fi lling adversely impact grain yield and its end-use quality for wheat. HTs strongly reduce the expression of major enzymes associated with starch synthesis, whereas enzymes associated with defence against stress and protein folding are dramatically increased. Using proteomics tools, the effect of different temperature regimes on storage protein (SP) accumulation was investigated. HT signifi cantly decreased the quantity per grain of individual gliadin and glutenin spots, but at maturity the ratio of gliadin to glutenin was not modifi ed. HT during grain fi lling strongly reduced starch accumulation, modifi ed the size distribution of starch granules, and to a much lesser extent, reduced the quantity of total proteins per grain. The aggregation and polymerisation of SP was investigated using asymmetric fl ow fi eld fl ow fractionation. Previous analyses of near-isogenic hard/soft lines showed that characteristics of glutenin polymers were signifi cantly infl uenced by puroindoline alleles ( Pina-D1a and -D1b ), and proteomics analysis showed that a typical mechanism of unfolded protein response occurs in ER, resulting from stress during protein accumulation. Effects of alleles encoding puroindolines, HMW-GS and LMW-GS, and temperature during grain development on glutenin polymer characteristics, dough rheological properties, and bread loaf volume were investigated for 40 cultivars grown in six environments in France. A difference of only 2 °C in average daily air temperature between locations during the grain-fi lling period resulted in increased molecular mass of the glutenin

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Section: High Temperature Is a Major Factor Affecting Wheat Productionmentioning

confidence: 99%

Coping with Wheat Quality in a Changing Environment: Proteomics Evidence for Stress Caused by Environmental Changes

Branlard

Lesage

Bancel

et al. 2015

Advances in Wheat Genetics: From Genome to Field

View full text Add to dashboard Cite

show abstract

“…on the physiology and biochemistry has been well investigated, there are only a few studies devoted to transcript profiling of wheat during high temperature stress (Altenbach & Kothari 2004;Altenbach et al 2007Altenbach et al , 2008. High temperatures reduce the duration of the developmental phases leading to fewer organs, smaller organs, and affect processes related to carbon assimilation.…”

Section: S95mentioning

confidence: 99%

Characterization and expression of high temperature stress responsive genes in bread wheat (Triticum aestivum L.)

Khurana¹,

Chauhan²,

Khurana³

2011

CZECH J GENET PLANT

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Abstract:To elucidate the effects of high temperatures, wheat plants (Triticum aestivum cv. CPAN 1676) were given heat shocks at 37°C and 42°C for two hours, and responsive genes were identified through PCR-Select Subtraction technology. Four subtractive cDNA libraries, including three forward and one reverse subtraction, were constructed from three different developmental stages. A total of 5500 ESTs were generated and 3516 high quality ESTs were submitted to Genbank. More than one third of the ESTs generated fall in unknown/no hit categories upon a homology search through BLAST analysis. A large number of high temperature responsive genes have been identified and characterized. Reverse subtraction analysis in developing grains showed extensive transcriptional changes upon heat stress as revealed by comparative analysis with forward subtraction. Differential expression was confirmed by cDNA macroarray and by northern/RT-PCR analysis. Expression analysis of wheat plants subjected to high temperature stress, after one and four days of recovery, showed fast recovery in seedling tissues. However, recovery was small in the developing seed tissue after two hours of heat stress. Ten selected genes were analysed in further detail by quantitative real-time PCR in an array of 35 different wheat tissues representing major developmental stages as well as different abiotic stresses. Tissue specificity was examined along with cross talk with other abiotic stresses and putative signalling molecules. The results obtained contribute towards understanding the regulation of genes at different developmental stages in wheat crucial to withstanding and recovery from heat stress.

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“…The conserved region, common to both classes is the barwin domain, named after the first characterized member of the PR-4 family, barley wound induced protein. The PR-4 genes are either developmentally regulated in cereal seeds, or induced by F. culmorum infection 47 , by Clonostachys rosea, a common worldwide saprophyte with a destructive effect against several plant pathogenic fungi 280 and by elevated temperatures 3 .…”

Section: Barwin (Barley Wound Induced Protein) (Pr-4)mentioning

confidence: 99%

“…In addition to a protective role against fungal pathogens, PR-4 may play a role in the response of the developing grain to elevated temperatures. Enhanced expression of wheatwin genes in seed produced under high temperature conditions may have important implications for wheat flour quality and its allergenic potential 3 . The presence of barwin in beer was recently established 118 .…”

Section: Barwin (Barley Wound Induced Protein) (Pr-4)mentioning

confidence: 99%

A Review: Biological and Technological Functions of Barley Seed Pathogenesis-Related Proteins (PRs)

Gorjanović

2009

Journal of the Institute of Brewing

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The mature barley seed proteome is dominated by proteins involved in stress responses, including the Pathogenesis-Related proteins (PRs). PRs are currently classified into 17 families. The biochemistry of the PRs families, whose members are constitutively present in barley seed, is surveyed in this paper. These proteins are assumed to protect dormant and germinating seeds against pathogenic microorganisms and pests. The current knowledge on PRs structural and functional properties is summarized in an attempt to illustrate their importance in barley seed innate resistance. At the same time, a platform to understand PRs technological function in cereal foods and in beverage processing and quality is provided.

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Genes encoding the PR-4 protein wheatwin are developmentally regulated in wheat grains and respond to high temperatures during grainfill

Cited by 26 publications

References 23 publications

Coping with Wheat Quality in a Changing Environment: Proteomics Evidence for Stress Caused by Environmental Changes

Coping with Wheat Quality in a Changing Environment: Proteomics Evidence for Stress Caused by Environmental Changes

Characterization and expression of high temperature stress responsive genes in bread wheat (Triticum aestivum L.)

A Review: Biological and Technological Functions of Barley Seed Pathogenesis-Related Proteins (PRs)

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