With the introduction of radioimmunoassay (RIA) techniques, it has become toxicologically possible to determine drug concentrations in postmortem vitreous humor. This study demonstrates and confirms this toxicological feasibility. In 49 medical examiner's drug related cases, postmortem tissue levels of morphine, barbiturates, and methadone were compared to the vitreous humor.

No abstract

This paper demonstrates that the RPTLC system can be used as a confirmation tool for regular adsorption TLC because it separates the pesticides of interest and also reverses their positions on the plate due to their partitioning with water solubility. A clinical procedure was devised for screening samples of gastric and urine for chlorinated, organophosphate, and carbamate insecticides via the use of conventional TLC. The presence of these insecticides was then confirmed by reverse-phase TLC via spotted TLC plates. In view of the plethora of insecticides currently found in the home, and in view of the numerous incidents of adults and children accidentally or purposely ingesting them. This confirming procedure should prove to be a clinically valuable tool in crucial emergency situations.

A procedure for separating barbiturates by reverse-phase TLC is described. A spotted TLC plate is dipped in mineral oil/petroleum ether (1:10) and run 15 cm in a solvent of water/methanol/ammonium hydroxide, (80:20:2). After visualization, the drugs tested showed distinct separation.