An antifungal metabolite, oosporein, was isolated from the culture of Verticillium psalliotae that produced the antagonistic effects on Phytophthora infestans. Oosporein exhibited a significant growth-inhibitory effect on P. infestans in comparison with other phytopathogenic fungi.
To determine the relationships between microbial biomass nitrogen (N), nitrate-nitrogen leaching and N uptake by plants, a field experiment and a soil column experiment were conducted. In the field experiment, microbial biomass N, 0.5 mol L −1 K 2 SO 4 extractable N (extractable N), NO 3 -N leaching and N uptake by corn were monitored in sawdust compost (SDC: 20 Mg ha ) and in soil treated with CF in 2001. Nitrogen was applied as (NH 4 ) 2 SO 4 at rates of 220 kg N ha −1 for SDC and SDC + RS treatments and at a rate of 300 kg N ha −1 for the CF treatment in both experiments. In the field experiment, microbial biomass N in the SDC treatment increased to 147 kg N ha −1 at 7 days after treatment (DAT) and was maintained at 60-70 kg N ha −1 after 30 days. Conversely, microbial biomass N in the CF treatment did not increase significantly. Extractable N in the surface soil increased immediately after treatment, but was found at lower levels in the SDC treatment compared to the CF treatment until 7 DAT. A small amount of NO 3 -N leaching was observed until 21 DAT and increased markedly from 27 to 42 DAT in the SDC and CF treatments. Cumulative NO 3 -N leaching in the CF treatment was 146 kg N ha −1 , which was equal to half of the applied N, but only 53 kg N ha −1 in the SDC treatment. In contrast, there was no significant difference between N uptake by corn in the SDC and CF treatments. In the soil column experiment, microbial biomass N in the SDC + RS treatment at 7 DAT increased with increased RS application. Conversely, extractable N at 7 DAT and cumulative NO 3 -N leaching until 42 DAT decreased with increased RS application. In both experiments, microbial biomass N was negatively correlated with extractable N at 7 DAT and cumulative NO 3 -N leaching until 42 DAT, and extractable N was positively correlated with cumulative NO 3 -N leaching. We concluded that microbial biomass N formation in the surface soil decreased extractable N and, consequently, contributed to decreasing NO 3 -N leaching without impacting negatively on N uptake by plants.
Clarifying the identity and enzymatic activities of microorganisms associated with the decomposition of organic materials is expected to contribute to the evaluation and improvement of composting processes. In this study, we examined the cellulolytic and hemicellulolytic abilities of bacteria isolated from sawdust compost (SDC) and coffee residue compost (CRC). Cellulolytic bacteria were isolated using Dubos mineral salt agar containing azurine cross-linked (AZCL) HE-cellulose. Bacterial identification was performed based on the sequence analysis of 16S rRNA genes, and cellulase, xylanase, β-glucanase, mannanase, and protease activities were characterized using insoluble AZCL-linked substrates. Eleven isolates were obtained from SDC and 10 isolates from CRC. DNA analysis indicated that the isolates from SDC and CRC belonged to the genera Streptomyces, Microbispora, and Paenibacillus, and the genera Streptomyces, Microbispora, and Cohnella, respectively. Microbispora was the most dominant genus in both compost types. All isolates, with the exception of two isolates lacking mannanase activity, showed cellulase, xylanase, β-glucanase, and mannanase activities. Based on enzyme activities expressed as the ratio of hydrolysis zone diameter to colony diameter, it was suggested that the species of Microbispora (SDCB8, SDCB9) and Paenibacillus (SDCB10, SDCB11) in SDC and Microbispora (CRCB2, CRCB6) and Cohnella (CRCB9, CRCB10) in CRC contribute to efficient cellulolytic and hemicellulolytic processes during composting.
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