Oxidative stress has emerged as a pivotal mechanism that underlies the toxic pulmonary effects of suspended particulate matter (SPM). Experimental evidence shows that redox-active transition metals, redox-cycling quinoids, and polycyclic aromatic hydrocarbons (PAHs) contained in SPM act synergistically, producing reactive oxygen species (ROS). The direct production of superoxide anion and the damaging hydroxyl radical has been studied in aqueous and dimethyl sulfoxide (DMSO) suspensions of SPM both with and without H2O2; however, no study has reported on the release of ROS from ingesting macrophages with SPM. We investigated the time course of the ability to induce lucigenin-dependent chemiluminescence (CL) from human monocyte-derived macrophages exposed to SPM, carbon black particles, and diesel exhaust particles (DEP). We also examined hydroxyl radical generation from the same experimental system using the 2-deoxy-d-robse method. We found an increase of CL for SPM, but not for carbon black particles or for DEP. Hydroxyl radical generation was observed in both SPM and DEP, but the release from DEP was more frequent than that from SPM. These results suggest that certain components of SPM are important in the response of ROS from ingesting macrophages with SPM, and that those components are discharged from SPM into the atmosphere.
Effect of the electrode materials on the resistive switching of Ti4O7 Appl. Phys. Lett. 101, 053502 (2012) Bipolar resistive switching characteristics of Al-doped zinc tin oxide for nonvolatile memory applications Appl. Phys. Lett. 101, 052901 (2012) Orbital symmetry induced conductance switching in a graphene nanoribbon heterojunction with different edge hydrogenations Appl. Phys. Lett. 101, 053101 (2012) Strongly coupled phase transition in ferroelectric/correlated electron oxide heterostructures Appl. Phys. Lett. 101, 042902 (2012) Electrical transition of (3,3) carbon nanotube on patterned hydrogen terminated Si(001)-2×1 driven by electric field
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.