SummaryChemostat cultures of carrot suspension cultures, where growth was limited by the concentration of phosphate in the input medium, were achieved by replacing a fixed proportion of the culture with fresh medium at daily intervals. In the range 0.05-0.30mM phosphate in the input medium and at a specific growth rate of 0.357 days-', steady-state culture density but not anthocyanin in the cells was strictly proportional to the input phosphate concentration with no intercept. At a phosphate concentration of O.lOmM and growth rates from 0.105 to 0.430 days-', the steadystate culture density could not be described by Monod's model of chemostat cultures, but could be descnied by Nyholm's model. The steady-state levels of anthocyanin were not strictly proportional to the steady-state biomass under all conditions, showing that anthocyanin production is not completely growth associated.

No abstract