BackgroundClinical evaluation to differentiate the characteristic features of pulmonary fibrosis and emphysema is often difficult in patients with combined pulmonary fibrosis and emphysema (CPFE), but diagnosis of pulmonary fibrosis is important for evaluating treatment options and the risk of acute exacerbation of interstitial pneumonia of such patients. As far as we know, it is the first report describing a correlation among clinical, radiological, and whole-lung pathological features in an autopsy cases of CPFE patients.MethodsExperts retrospectively reviewed the clinical charts and examined chest computed tomography (CT) images and pathological findings of an autopsy series of 22 CPFE patients, and compared these with findings from 8 idiopathic pulmonary fibrosis (IPF) patients and 17 emphysema-alone patients.ResultsAll patients had a history of heavy smoking. Forced expiratory volume in 1 s/forced vital capacity (FEV1/FVC%) was significantly lower in the emphysema-alone group than the CPFE and IPF-alone groups. The percent predicted diffusing capacity of the lung for carbon monoxide (DLCO%) was significantly lower in the CPFE group than the IPF- and emphysema-alone groups. Usual interstitial pneumonia (UIP) pattern was observed radiologically in 15 (68.2%) CPFE and 8 (100%) IPF-alone patients and was pathologically observed in all patients from both groups. Pathologically thick-cystic lesions involving one or more acini with dense wall fibrosis and occasional fibroblastic foci surrounded by honeycombing and normal alveoli were confirmed by post-mortem observation as thick-walled cystic lesions (TWCLs). Emphysematous destruction and enlargement of membranous and respiratory bronchioles with fibrosis were observed in the TWCLs. The cystic lesions were always larger than the cysts of honeycombing. The prevalence of both radiological and pathological TWCLs was 72.7% among CPFE patients, but no such lesions were observed in patients with IPF or emphysema alone (p = 0.001). The extent of emphysema in CPFE patients with TWCLs was greater than that in patients without such lesions. Honeycombing with emphysema was also observed in 11 CPFE patients.ConclusionsTWCLs were only observed in the CPFE patients. They were classified as lesions with coexistent fibrosing interstitial pneumonia and emphysema, and should be considered an important pathological and radiological feature of CPFE.

These findings suggested that bFGF-induced collateral development exceeded VEGF-induced collateral development in the induction of arteriogenesis, and that combined gene delivery of VEGF and bFGF produced additive or synergistic effects of collateral development as compared with the effects induced by transfer of each gene alone.

This study was performed to assess the utility of tumor-derived fragmentary DNA, or circulating tumor DNA (ctDNA), for identifying high-risk patients for relapse of acute myeloid leukemia and myelodysplastic syndrome (AML/MDS) after undergoing myeloablative allogeneic hematopoietic stem cell transplantation (alloSCT). We retrospectively collected tumor and available matched serum samples at diagnosis and 1 and 3 months post-alloSCT from 53 patients with AML/MDS. After identifying driver mutations in 51 patients using next-generation sequencing, we designed at least 1 personalized digital polymerase chain reaction assay per case. Diagnostic ctDNA and matched tumor DNA exhibited excellent correlations with variant allele frequencies. Sixteen patients relapsed after a median of 7 months post-alloSCT. Both mutation persistence (MP) in bone marrow (BM) at 1 and 3 months post-alloSCT and corresponding ctDNA persistence (CP) in the matched serum (MP1 and MP3; CP1 and CP3, respectively) were comparably associated with higher 3-year cumulative incidence of relapse (CIR) rates (MP1 vs non-MP1, 72.9% vs 13.8% [P = .0012]; CP1 vs non-CP1, 65.6% vs 9.0% [P = .0002]; MP3 vs non-MP3, 80% vs 11.6% [P = .0002]; CP3 vs non-CP3, 71.4% vs 8.4% [P < .0001]). We subsequently evaluated whether subset analysis of patients with 3 genes associated with clonal hematopoiesis, DNMT3A, TET2, and ASXL1 (DTA), could also be helpful in relapse prediction. As a result, CP based on DTA gene mutations also had the prognostic effect on CIR. These results, for the first time, support the utility of ctDNA as a noninvasive prognostic biomarker in patients with AML/MDS undergoing alloSCT.

and near the Asamushi Marine Biological Station, Tohoku University, Aomori, Japan. Naturally spawned eggs were fertilized with a suspension of non-self sperm. Embryos were cultured at 9-13°C. Tadpole larvae hatched after 35 hours of development at 13°C. Isolation and dissociation of blastomeresEggs were manually devitellinated with sharpened tungsten needles, and reared in 0.9% agar-coated plastic dishes filled with sea water. Blastomeres were identified and isolated from embryos by use of a fine glass needle under a stereomicroscope. Isolated blastomeres were cultured separately in agar-coated plastic dishes. Partial

Clinically significant and palpable enlargement of the gastrocnemio-semimembranosus bursa is known as a Baker's cyst. Baker's cysts may rupture, resulting in a swollen, painful leg that is clinically indistinguishable from acute deep vein thrombosis. For this reason, ruptured Baker's cysts are sometimes called pseudothrombophlebitis. The purpose of this study was to determine the incidence of ruptured Baker's cysts, and to evaluate the role of ultrasonography in the diagnosis of this condition. The hospital records of 106 patients (43 men and 63 women) who were referred to the vascular surgical department at Saitama Medical Center with unilateral or bilateral swollen legs between June 1997 and June 2000 were reviewed retrospectively. The total number of affected limbs was 125, being 52 right legs and 73 left legs. Deep vein thrombosis was the most common cause of swollen legs, being diagnosed in 44.8%. No specific anatomical derangement was found in 39 limbs (31.2%), and these were defined as idiopathic. Lymphedema was also common, being diagnosed in 16 limbs. Ruptured Baker's cysts were observed in three limbs, with an incidence of 2.4%. In all of these patients, a large hypoechoic space was seen behind the calf muscles and this sonolucent area was easily detected by a conventional scanner, being pathognomonic of a ruptured Baker's cyst.