Background: Cervical cancer is one of the common malignant tumors in women, among which squamous cell carcinoma of the cervix accounts for more than 90%. Currently, 30% of patients with intermediate to advanced squamous cervical cancer are still uncontrolled or recurrent after standard radical simultaneous radiotherapy; therefore, the search for key genes affecting the sensitivity of radiotherapy may lead to new strategies for treatment.Methods: Differentially expressed genes were identified by GEO2R from the gene expression omnibus (GEO) website, prognosis-related genes for cervical cancer were obtained from other databases, then gene ontology (GO) was analyzed by DAVID. Meanwhile, protein-protein interaction network was constructed by STRING, By Online analysis of differentially expressed genes, prognostic genes, and CCDB(cervical cancer gene database website: http://crdd.osdd.net/raghava/ccdb/index.php) through the OncoLnc database, we aim to search for differentially expressed genes associated with prognosis validation by immunohistochemistry.Result: 298 differentially expressed genes, 724 genes associated with prognosis were found. Eventually, 2 genes, ASPH and NKAPP1, were identified through validation as genes that affect both sensitivities to radiotherapy and survival. Then, immunohistochemical results showed that the ASPH gene was indeed highly expressed in the radiotherapy-resistant group and had lower OS and PFS.Conclusion: This study aims to better understand the characteristics of cervical cancer radiation therapy resistance-related genes through bioinformatics, and provide further research ideas for finding new mechanisms and potential therapeutic targets related to cervical cancer radiation therapy.
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