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In this experiment, the effect of excessive exercise on the sexual cycle and histological changes of various organs of albino rats were observed. Of the sexual cycle of albino rats Gotoda (1) found that the fatigue caused by vibration brings about a considerable prolongation of the anestrus period. Hirai (2) made a comparative histological study on the myocard and skeletal muscle of animals which habitually move actively and those which do not move so much.Ogawa (3) made albino rats swin and measured changes in the weight of the principal organs.Selye (4, 5) investigated histological changes in each stage of stress reaction and reported that the hypersecretion of the adrenal cortical hormone and pituitary ACTH, hemorrhagic ulcer in stomach and intestine, nodular myocarditis, perivasculitis, abnormal swelling of renal tubuli and the degenerative change of thymus could be seen. METHODExperiments were made on albino rats weighing 120-150g. using a method requiring forced swimming in a tank.Tests were made in the following three groups:A group (excessively trained):trained for 60 or over 90 days. B group (castrated and excessively trained):4 weeks after castration, 60 days training was applied. C group (untrained):untrained rats were forced-swum. For the test of the sexual cycle, 36 rats were selected whose sexual cycle (vaginal smear method) were comparatively regular. At first they were given light exercise of 10-30 minutes swimming and gradually the time was extended and by 17 days the training lasted 3-6 hours daily.The vaginal smear was examined before the exercise and if deemed necessary several times a day at an interval of two hours.The sexual cycle was divided into 4 phases according to Zondek and Aschheim; prestrus (P), estrus (E), metestrus (M) and diestrus (D).Histological changes in various organs were observed under ordinary staining, but according to necessity the fat, hemosiderin and calcium were examined under special staining.

Summary a) In the course of our polygraphic study of sleep, a new method of EEG analysis was developed. As the result of a simple modification of the ordinary Walter type frequency analyser. we succeeded in obtaining a compact and easily observable record showing the long time variation of the integrated values of each band respectively. b) The application of this method to the study of sleep EEG opened a new aspect. 1. The most remarkable feature of this analysis is the undulatory fluctuation of relatively long time in the low frequency component (long undulatory fluctuation), especially in 1–2 cps band. In general, one sleep cycle is composed of a few of these undulatory fluctuations repeatedly appearing and the state of slight fluctuation mainly corresponding to the paradoxical phase. This long undulatory fluctuation corresponds to successive sleep phases in certain rule. The gradual change appearing at the time of a shift from the moderate phase to the deep phase is characteristic. 2. Generally the crest of the highest of these undulatory fluctuations during successive sleep cycles is highest at the first cycle and tends to decrease toward morning. The increasing slope is steepest at the first cycle and gentler toward morning. 3. During the ascending stage of this undulatory fluctuation the gross body movements are very few and in the other periods are relatively many. Most of the abrupt decreases of the integrated values of the low frequency band are accompanid by gross body movement. This report is dedicated to Professor Haruo Akimoto, Director of the Department of Neuropsychiatry, Faculty of Medicine, University of Tokyo, in commemoration of his sixtieth birthday. This study was carried out with the earnest cooperation of C. Sasaki, B.A. and N. Nagamura, M.A. We are much indebted to Mr. K. Akagi of San'ei Instrument Ltd. for the technical support.