A new type of bleomycin group antibiotic, SF-1961 was isolated from the fermentation broth of Streptomyces species. SF-1961 possessed B-amino-B-(4-amino-6-carboxypyrimidin-2-yl)-propionic acid instead of its 5-methyl derivative which was a common component of bleomycins and phleomycins.In our screening studies of new antibiotics, a novel bleomycin group antibiotic was isolated from the culture filtrate of Streptomyces sp. SF-1961, which was isolated from a soil sample collected at Himeji, Hyogo, Japan. In this paper, taxonomy of the strain, isolation and characterization of the antibiotic are described.
Taxonomic StudiesThe taxonomic characterization of the strain SF-1961 was carried out by the same method as described in the preceding paper". The microorganism was identified as a strain of Streptomyces A well-grown agar slant of the producing organism was used to inoculate to the seed medium containing 2.0% soluble starch, 1.0% Polypeptone, 0.3% beef extract and 0.05% K2HPO4. After incubation for 20 hours at 28°C, the first seed culture (1 %) was inoculated into five shaking flasks each containing 100 ml of medium to prepare the second seed culture. The second seed culture was then transferred into a 30-liter jar fermentor for 20 hours, 10 liters of the third culture were seeded into 200 liters of medium containing 2.5 % glycerol, 2.5 % soybean meal, 0.5 % fish meal, 0.25 % NaCl and 0.0005% CuSO4 •5H2O in a 300-liter tank. The fermentation was carried out at 28°C for 20 hours and then at 24°C for an additional 70 hours.
A new aminoglycoside antibiotic, dactimicin produced by a Dactylosporangium matsuzakiense SF-2052 has been isolated by column chromatography on a cation-exchange resin and CM-Sephadex. The structure of dactimicin was determined to be 4-amino-l,4-dideoxy-3-O-(2,6-diamino-2,3,4,6,7-pentadeoxy-L-lyxo-heptopyranosyl)-1-[(N-formimidoylglycyl)methylamino]-6-O-methyl-L-chiro-inositol.
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