Objectives: This study tests, for the first time, the applicability of a new method of sex estimation utilizing enamel peptides on a sample of deciduous and permanent teeth at different stages of mineralization, from nonadults of unknown sex, including perinates. Materials and methods: A total of 43 teeth from 29 nonadult individuals aged from 40 gestational weeks to 19 years old were analyzed. The sample included pairs of fully mineralized and just developing teeth from the same individual. The individuals were from four archaeological sites in England: Piddington (1st-2nd centuries AD), Coach Lane, Victoria Gate, and Fewston (all 18th-19th centuries). A method that identifies sex chromosome-linked isoforms of the peptide amelogenin from human tooth enamel was applied. The method utilizes a minimally destructive acid etching procedure and subsequent nano liquid chromatography tandem mass spectrometry. Results: It was possible to determine the sex of 28 of the nonadult individuals sampled (males = 20, females = 8, undetermined = 1). Only one sample failed (CL9), due to insufficient mineralization of the sampled tooth enamel. Data are available via ProteomeXchange with identifier PXD021683. Discussion: Sufficient peptide material to determine sex can be recovered even from the crowns of developing perinatal teeth that are not fully mineralized. The minimally destructive and inexpensive (compared to ancient DNA) nature of this procedure has significant implications for bioarchaeological studies of infancy and childhood.
Aims and Objectives Macroscopic skeletal analysis and stable carbon and nitrogen isotope analysis were employed to examine the relationship between skeletal “stress” lesions and changes in the isotopic life history profiles of six nonadults from the Gepid population buried at the Archiud “Hânsuri” Cemetery (4th–7th centuries AD). Materials and Methods The Gepids were a migratory barbarian population in the Carpathian Mountain basin of Transylvania, Romania. Macroscopic skeletal analysis was conducted on 32 individuals, and of those, six nonadults were selected for stable carbon and nitrogen isotope analysis of incrementally sampled dentine. Results Macroscopic skeletal analyses revealed 47% of the analysed population displayed evidence of childhood stress. Stable carbon and nitrogen isotope ranges were −17.7‰ to −11.8‰ for δ13C and 9.4‰ to 15.1‰ for δ15N. Discussion The overall dietary profile indicates a mixed terrestrial diet (C3/C4) with increased consumption of C4 plants during adolescence. The six nonadults appear to have been breastfed from 1 to 6 months and weaned by 3 years of age. High δ15N values seen in pre‐natal and post‐natal increments may suggest a level of nutritional/physiological stress during gestation and during the transitions from umbilical nutrients, breastmilk, and weaning foods. Although limited by the small sample size, this study supports the link between elevated δ15N values and nutritional stress and the relationship and timing of skeletal lesions with changes in the isotope profiles and was among the first to combine palaeopathological analyses and incremental stable isotope analyses on the Transylvania Gepids.
Drawing on the results of new multi-method research in Grotta Regina Margherita-the largest known Middle Bronze Age mortuary cave in west-central Italy (ca. 1650-1450 B.C.)-this article helps to replace the generic idea of "collective burial" with a more precise understanding of how the bodies of the deceased were transformed into potent social, symbolic, and sensuous resources housed in caves. It contextualizes this process within a nuanced understanding of settlement and subsistence practices, in which relatively short-lived and small-scale agricultural communities extended inland to the edge of the Apennine Mountains, ritually demarcating mortuary assemblages in caves in the process.
Metastases arise from a subset of cancer cells that disseminate from the primary tumor; however, the factors that contribute to proliferation of cancer cells in a secondary site are incompletely understood. The ability of cancer cells to thrive in a new tissue site is influenced by genetic and epigenetic changes that are important for disease initiation and progression, but these factors alone do not predict if and where cancers metastasize. Specific cancer types metastasize to consistent subsets of tissues, suggesting that factors within the primary tumor influence the tissue environments where cancers can grow. Using pancreatic cancer as a model, we find that primary and metastatic tumors are metabolically similar to each other and that the tumor initiating capacity and proliferation of both primary- and metastasis-derived cells is favored in the primary site relative to the metastatic site. Moreover, propagating lung or liver metastatic cells in vivo to enrich for tumor cells adapted to grow in the lung or the liver does not enhance their relative ability to form large tumors in those sites, change their preference to grow in the primary site, nor stably alter their metabolism relative to primary tumors. To assess whether this preference for the primary site is specific to pancreatic cancer, we analyzed liver and lung cancer cells and find that these cells also best form tumors in the tissue that corresponds to their primary site. Together, these data suggest that the cancer tissue-of-origin influences the metabolism of both primary and metastatic tumors and may impact whether cancer cells can thrive in a metastatic site.
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