A cell-free system for synthesizing protein from wheat germ was used to translate the messenger RNA extracted from 16-day embryonic chick calvaria. A part of the product had properties similar to collagenous peptides and served as a substrate for prolyl hydroxylase, an enzyme specific for collagen. The level of potassium was critical for the synthesis of high molecular weight products with properties similar to pro-alpha-chains. The potassium concentration for optimal protein synthesis, as judged by maximum incorporation of [3H]proline into acid precipitable material, was considerably lower than the concentration required for the synthesis of high molecular weight collagenous peptides.
This study showed that the initiation of granuloma formation in alcoholic mice was retarded but eventually reached normal levels. The delay appears to be related to a failure of cells to migrate into the sponge as rapidly as they did in control animals. Thus, there is clear evidence to support the clinical impression of poor wound healing in alcoholics. Although repair is eventually accomplished, the initial decrease in the rate of this process may be significant enough to put the alcoholic patient at risk.
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