Heterogeneity in Keratoconus: Possible Biochemical Basis

Yue, Beatrice Y.J.T.; Sugar, Joel; Benveniste, Kathy

doi:10.3181/00379727-175-41804

Cited by 50 publications

(29 citation statements)

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“…As the disease progresses, there is a variability between keratoconus corneas in terms of the relationship between the severity of the disease and the extent of the structural alterations. Irrespective of the initial pathogenesis, which probably involves an up-regulation of degradative enzymes (Kenney et al, 1994;Kenney and Brown, 2003;Sawaguchi et al, 1989;Zhou et al, 1998) leading to some loss of collagen (Yue et al, 1984), the findings presented in this paper support a mechanism of tissue thinning in keratoconus corneas that also involves lamellar or fibrillar slippage. This idea is supported by the observation of abnormal collageneproteoglycan arrangement in keratoconus corneas (Fullwood et al, 1990(Fullwood et al, , 1992.…”

Section: Discussionsupporting

confidence: 48%

A study of corneal thickness, shape and collagen organisation in keratoconus using videokeratography and X-ray scattering techniques

Hayes

Boote

Tuft

et al. 2007

Experimental Eye Research

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In keratoconus, the cornea becomes progressively ectactic resulting in severe visual impairment. Here, we use a combination of videokeratography and synchrotron X-ray diffraction to investigate the relationship between corneal shape and thickness, and the distribution and predominant orientation of stromal fibrillar collagen in five keratoconus corneas. In all but the least advanced case, the thinning and ectasia measured in vivo using corneal videokeratography was accompanied by corresponding changes in the relative distribution and orientation of stromal collagen in the excised corneal buttons. Although the most severe case of keratoconus possessed the most pronounced stromal collagen alterations, and only a minor disruption to stromal collagen arrangement was seen in the least advanced case, a variability in the extent of stromal collagen alteration was seen between these clinical extremes. The observed abnormalities in collagen distribution and orientation are consistent with a mechanism of keratoconus progression that involves inter-fibrillar or inter-lamellar slippage causing a redistribution of tissue within the cornea.

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Section: Discussionsupporting

confidence: 48%

A study of corneal thickness, shape and collagen organisation in keratoconus using videokeratography and X-ray scattering techniques

Hayes

Boote

Tuft

et al. 2007

Experimental Eye Research

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“…Since collagen is responsible for the strength and rigidity of the cornea, one attractive hy pothesis is that KC results from a defect in collagen or some other extracellular matrix Variations in solubilization procedures and the efficiency of extraction could account for the conflicting reports in the literature. Pre vious studies have also provided evidence that KC may be a heterogeneous disease [13,14]. However, the basis of this heterogeneity has not been identified.…”

Section: Introductionmentioning

confidence: 99%

Heterogeneity of Type I Collagen Expression in Human Corneal Keratoconus Fibroblasts

Peters¹,

Harrison

Brandt

1993

Ophthalmic Res

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Corneal fibroblast cultures were established from 3 normal and 4 keratoconus (KC) corneas. Type I collagen protein synthesis and steady-state RNAs were analyzed in these cultures by metabolic labeling studies, slot blots and immunofiuorescent microscopy. Type I collagen proteins and steady-state RNAs were reduced in cells from 3 of the 4 KC cultures when compared with normal fibroblasts, suggesting that collagen expression is heterogenous in KC and that the heterogeneity is expressed at the cellular and molecular level. The type I collagen matrix synthesized by the KC fibroblasts appeared normal when analyzed by immunofluorescence, suggesting that the incorporation of type I collagen into the extracellular matrix is not affected in KC fibroblasts.

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“…74 In vivo confocal microscopy can provide greater insight into the morphological changes that occur with this progressive noninflammatory corneal ectasia. By improving our understanding of keratoconus this may enable alternative therapies to be devised in order to retard its progression and therefore reduce the need for surgical intervention.…”

Section: 39mentioning

confidence: 99%

Quantitative analysis of corneal microstructure in keratoconus utilising in vivo confocal microscopy

Weed

MacEwen

Cox

et al. 2006

Eye

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Heterogeneity in Keratoconus: Possible Biochemical Basis

Cited by 50 publications

References 0 publications

A study of corneal thickness, shape and collagen organisation in keratoconus using videokeratography and X-ray scattering techniques

A study of corneal thickness, shape and collagen organisation in keratoconus using videokeratography and X-ray scattering techniques

Heterogeneity of Type I Collagen Expression in Human Corneal Keratoconus Fibroblasts

Quantitative analysis of corneal microstructure in keratoconus utilising in vivo confocal microscopy

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