A Gram-stain-variable, rod-shaped, motile bacterial strain, designated S1-20 T , was isolated from marine sediment of the Yellow Sea in China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S1-20 T was related to the genus Virgibacillus and had highest 16S rRNA gene sequence similarities with the type strains of Virgibacillus necropolis (98.1 %), Virgibacillus arcticus (97.7 %) and Virgibacillus carmonensis (97.3 %). The predominant cellular fatty acids of strain S1-20 T were anteiso-C 15 : 0 and anteiso-C 17 : 0 . The main menaquinone was MK-7. The genomic DNA G+C content of strain S1-20 T was 38.3 mol%. The polar lipids profile contained diphosphatidylglycerol, phosphatidylglycerol, five unidentified phospholipids, one unknown aminophospholipid and an aminolipid. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain S1-20 T is a representative of a novel species of the genus Virgibacillus, for which the name Virgibacillus flavescens sp. nov. is proposed. The type strain is S1-20 T (5LMG 28381 T 5DSM 29015 T ).
The Gram-stain-negative, rod-shaped bacterial strain designated RU-1-R-18T was isolated from intertidal sediment on Sakhalin Island in Russia. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RU-1-R-18 T was related to the genus Puniceibacterium and shared highest sequence similarities with the type strain Puniceibacterium antarcticum KACC 16875 T (97.9 %). The predominant cellular fatty acid was C 18 : 1 v7c. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unidentified aminophospholipid and seven unidentified polar lipids. The genomic DNA G+C content of strain RU-1-R-18 T was 59.1 mol%. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain RU-1-R-18 T
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