Thymosm(Ti) is a 4.9 kDa polypeptide that interacts with G-actin and is thought to be an important mediator in cell proliferation, migration, and differentiation. T4 has been identified as a factor involved in the differentiation of human umbilical vein endothelial cells (HUVECs) cultured on Matrigel. Here we have used various in vitro and in vivo migration assays to demonstrate the role of T134 in endothelial cell migration. Our results demonstrate that T4 acts as a chemoattractant for endothelial cells, stimulating the migration of HUVECs in Boyden chambers four-to sixfold over that observed with media alone. Of the primary cell types tested, only human coronary artery cells responded to T4 treatment, suggesting that the migration activity of T(34 was endothelial cell-specific. Tf4 significantly accelerated the rate of migration into the scratch wounded area of a HUVEC monolayer.
Four adolescents had basilar migraine, infrequent cerebral seizures, and severe EEG abnormalities. The clinical course was benign, with normal personality, mentation, and neurologic examination. Almost continuous EEG abnormalities, consisting of rhythmic temporo-occipital sharp and slow wave discharges, or generalized spike and wave complexes, were seen in serial recordings. The rare seizures, either focal or generalized, usually followed a migrainous aura and seemed to be readily controlled with anticonvulsant medication. The complex relationship between classical migraine and epilepsy is illustrated by this syndrome; recognition of its relatively benign course may prevent unnecessary investigation and undue alarm.
Plasma levels of 14 amino acids were determined in 44 probands with 3/sec spike-wave epilepsy, 27 of their first-degree relatives, and 22 controls. Six ratios of metabolically related amino acids were also calculated. Statistically significant differences were found for 7/20 variables when the experimental and control probands were compared, and for 6/20 variables when the relatives were compared with controls. Tau, Asp, and Tau/Glu were decreased and Glu was was increased in both the experimental probands and their first degree relatives. When discriminant analysis was used, age- and sex-matched epileptic probands could be distinguished from the age- and sex-matched control probands with 100% accuracy employing only 3 amino acids (Asp, Glu, Val) and one ratio (Thr/Ser).
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