Background and Aim: Cynomolgus monkeys (Macaca fascicularis) develop spontaneous infection of Papillomavirus (PV); thus, potentially beneficial for modeling human PV (HPV) infection study. Contrary to human origin, infection in cynomolgus monkeys does not always show evident clinical symptoms of cervical cancer. The absence of cervical cancer clinical symptoms leads us to investigate the molecular mechanism of the HPV infection in cynomolgus monkeys. This study aimed to investigate the messenger ribonucleic acid (mRNA) expression levels of KI67 and P53 genes, majorly known as biomarker oncogenesis of PV infection. Materials and Methods: The polymerase chain reaction (PCR) technique was used with MY11/MY09 primer to screen PV in cynomolgus monkey, further grouped as positive-PV and negative-PV infection groups. Real-time quantitative PCR was also applied to quantify the mRNA expression levels of KI67 and P53 genes in animals. Results: Increased expression of mRNA level of KI67 genes was significantly higher in Positive- PV group than negative-PV group. In contrast, the P53 mRNA expression level increased markedly higher in the negative-PV group than in the positive-PV group. Conclusion: Our study describes the potential of cynomolgus monkeys as a spontaneous oncogenesis model of PV infection-type. However, we used a limited number of cancer genetic markers. So, further study of other genetic markers is required to prove that cervical cancer could be developed naturally in cynomolgus monkeys.
AbstrakStudi ini bertujuan untuk menganalisis faktor-faktor yang mempengaruhi ekspor kayu lapis dengan menggunakan Gravity Model, dan menghitung Nilai Tarif Ekuivalen dari Non Tariff Barriers (NTBs) kayu lapis Indonesia di negara tujuan. Berdasarkan pendekatan Gravity Model (Model Gravitasi), aliran perdagangan potensial diperoleh dengan melakukan subtitusi seluruh data kedalam persamaan gravity. Fitted trade flow dari persamaan gravity model dianggap sebagai aliran perdagangan potensial. Perbedaan antara aliran perdagangan aktual dan potensial diindikasikan sebagai tarif ekuivalen dari NTBs. Hasil estimasi menunjukkan faktor-faktor yang berpengaruh terhadap ekspor kayu lapis Indonesia yaitu Gross Domestic Product (GDP) riil Indonesia dan negara tujuan, Indek Harga Konsumen (IHK) Indonesia dan negara tujuan, jarak ekonomi, nilai tukar dan krisis keuangan tahun 2010. Hasil perhitungan menunjukkan negara Uni Eropa seperti Inggris dan Belgia memiliki rata-rata tarif ekuivalen NTBs paling besar. Sistem Verifikasi Legalitas Kayu (SVLK) untuk legalitas produk kayu dapat dijadikan sebagai strategi untuk menghadapi NTBs yang ada di negara tujuan ekspor. Kata Kunci: Non-Tariff Barries (NTBs), Gravity Model, Volume Ekspor, Data Panel Abstract This study sets out to analyze factors that influence plywood exports utilizing the Gravity Model and assess the equivalent tarriff value of Non Trade Barriers plywood on destination countries. Based on the Gravity Model approach, a potential of flow of trade is obtained through subsituting the whole data into gravity equation. Fitted trade flow from the Gravity Model equation is considered as a potential trade flow. The difference between actual and potential trade flow is indicated by the NTB equivalent tariff. The result shows factors that are influential on plywood exports notably the GDPs of Indonesia and destination countries, Consumer Index Price in
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