(Dnline) 2 Materials and methods 2.1 Samples Samples of the Fuji Suprema and Lis Gala apple varieties were harvested at the Experimental Station of the Agricultural and Rural Extension (EPAGRO) in Santa Catarina, Brazil (26° 46' 31" S, 51° 00' 54" D). The Gala variety was harvested from Boutin Agricola Company in Paraná, Brazil (25° 32' 08" S, 49° 53' 33" D). The varieties were chosen based on world apple production. Gala, Fuji and their clones are among the five most produced varieties in the world. The fruits were harvested (20 kg) at three ripening stages (unripe, ripe and senescent) from different cardinal points, and from the top and the bottom of six trees. The maturation index was determined by using the Starch-iodine test according Blanpied & Silsby (1992) using an
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