Abstract.Open tubular columns were coated with Chirasil-Dex, a polysiloxane anchored permethylated /3-cyclodextrin stationary phase. A number of chiral separations of pharmaceuticals and agrichemicals are presented to demonstrate the usefulness of this stationary phase in both GC and SFC. Racemates of various amines, amino acids, amino alcohols, carboxylic acids, coumarines, diols, and imides were resolved. To achieve acceptable separation of some enantiomers, a series of derivatives were studied. In many cases, cyclic derivatives and free acids gave the highest enantioselectivity.
Application of cellulose-based chiral stationary phases was extended to open tubular columns. These chiral materials were mixed with achiral matrix stationary phases. Compromises were found among the polarity and the ratio of achiral matrix polymers against the content of the chiral cellulose derivative in order to optimize the resolution of the investigated racemates. In GC, the high efficiency feature of open tubular columns allows fast analysis, however, compounds which express strong H-bond interaction with cellulose derivatives elute with a bad peak shape. The application of these stationary phases for open tubular SFC was more successful, because the solvation power of the mobile phase can compensate the strong interaction between the solute and the cellulose derivative. Immobilization of the stationary phases were achieved for SFC purposes.
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