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Five long-term tillage studies in Kansas were evaluated for changes in soil properties including soil organic carbon (SOC), water holding capacity (WHC), bulk density, and aggregate stability. The average length of time these studies have been conducted was 23 yr. Soil properties were characterized in three depth increments to 30 cm, yet changes due to tillage, N fertility, or crop rotation were found primarily in the upper 0-to 5-cm depth. Decreased tillage intensity, increased N fertilization, and crop rotations that included cereal crops had greater SOC in the 0-to 5-cm soil depth. Only one of five sites had greater WHC, which occurred in the 0-to 5-cm depth. Aggregate stability was highly correlated with SOC at all sites. No-tillage (NT) had greater bulk density, but values remained below that considered root limiting. Soil organic C levels can be modified by management that can improve aggregate stability, but greater SOC did not result in greater WHC for the majority of soils evaluated in this study. MATERIALS AND METHODS Five long-term study sites were selected across the state of Kansas as described in Tables 1 and 2, and located in Fig. 1.

The use of no‐tillage has notably increased in the Pampas region of Argentina during the last 10 yr. Two tillage experiments with contrasting previous agricultural use, degraded and non‐degraded soils, were evaluated in the southeast of Buenos Aires province, Argentina. The objectives were to: (i) quantify the effects of tillage and N fertilization on quantity and vertical distribution of C and N in the soil organic matter (SOM) and particulate organic matter (POM) fractions as well as potentially mineralizable N (PMN), and (ii) evaluate these fractions as indicators of soil quality. Tillage systems were conventional tillage (CT), minimum tillage (MT), and no‐tillage (NT) (main plots), and N fertilization rates were 0, 120, and 150 kg ha−1 (subplots). Total organic C (TOC), total N (TN), POM‐C, POM‐N, and PMN were measured at 0‐ to 7.5‐ and 7.5‐ to 15‐cm soil depth. In Exp. I (degraded soil) TOC was greater under NT (27 g kg−1) than under CT (24 g kg−1) in the 0‐N treatments. No differences in TOC and TN were found in Exp. II at 0 to 7.5 cm (non‐degraded soil). Carbon in POM and POM‐N were greater under NT in the fractions of 212 to 2000 and 53 to 212 μm at 0 to 7.5 cm, but they were similar or greater under CT at 7.5‐ to 15‐cm depth in Exp. I. Stratification of TOC, TN, and POM were observed under NT in Exp. I. Potentially mineralizable N was greater under NT (62 mg kg−1) in Exp. I, however, no differences in PMN were observed in Exp. II. Carbon in POM 212 to 2000 μm and PMN were the more sensitive indicators of tillage effects, mainly in Exp. I.

Soil carbon sequestration is a viable shortterm option to mitigate increased atmospheric CO 2 . In agriculture, strategies to increase the soil carbon (C) sink include no-tillage, cover crops, and improved crop rotation. The objective of this study was to determine the influence of tillage systems on SOC and total N, soil aggregation and aggregate associated C and N in three soil types: Oxisol (Brazil), Vertisol (Argentina), and Mollisol (USA). Long-term tillage experiments included tilled (T) and no-till (NT) systems. A native grassland was included for comparison in each site. Soil samples were taken at 0-5, 0-15, and 15-30 cm depths.Water-stable aggregates (WSA) were separated using a wet-sieving method. Total C and total N were determined by dry combustion. A shift from native grassland to an agroecosystem decreased microbial biomass, but this decrease was less pronounced under NT. Cultivation reduced the mass of macroaggregates and the concentration associated C and N; however among agroecosystems, NT, regardless soil type, tended to be more similar to the native grassland sites. Agroecosystems reduced TOC and total N stocks, regardless of soil type, compared to the native grassland. This effect followed: Mollisol [ Oxisol [ Vertisol, and was more pronounced at the 0-5 cm soil depth than at deeper depths. This loss of C and N was associated with the decrease in the mass of macroaggregates and lower C and N concentrations of the aggregates. Macroaggregation was related to TOC and microbial biomass in the Mollisol, suggesting that the biological process of aggregate formation is the principal mechanism of C protection in these soils. The relationship between TOC and large macroaggregates showed lower values for the Oxisol and Vertisol, indicating that in these soils TOC has a complementary role in macroaggregation.

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To date, no studies have evaluated nitrous oxide (NO) emissions of a single versus a split-nitrogen (N) fertilizer application under different soil drainage conditions for corn ( L.). The objective of this study was to quantify season-long cumulative NO emissions, N use efficiency, and soil N dynamics when corn received a recommended N rate as single or split-N application in Minnesota soils with and without tile drainage over two growing seasons. Preplant urea was broadcast incorporated, and in-season split-N was broadcast as urea plus urease inhibitor. Tile drainage reduced NO emissions during periods of excess moisture but did not affect grain yield or other agronomic parameters. Conversely, when precipitation was adequate and well distributed, tile drainage did not affect NO emissions, but it did enhance grain yield. Averaged across years, the undrained soil emitted 1.8 times more NO than the drained soil (2.36 vs. 1.29 kg N ha). Compared with the Zero-N control, the Single Preplant and Split N applications emitted 2.1 and 1.6 times more NO, produced 1.4 and 1.3 times greater grain yield, and resulted in 1.5 and 1.4 times more residual soil total inorganic N, respectively. Per unit of grain yield, the Split application emitted similar amounts of NO as the Zero-N control. Averaged across years and drainage, the Split application emitted 26% less NO than the Single Preplant application (1.84 vs. 2.48 kg N ha; < 0.001) with no grain yield differences. These results highlight that soil drainage can reduce NO emissions and that a split N application may be a feasible way to achieve NO reduction while enhancing grain yield.

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Alfalfa is recommended as a rotational crop in corn production, due to its ability to contribute to soil nitrogen (N) and carbon (C) stocks through atmospheric N2fixation and above- and belowground biomass production. However, there is little information on how alfalfa management practices affect contributions to soil and subsequent corn crop yields, and research has not been targeted to organic systems. A study was conducted to determine the effects of alfalfa stand age, cutting frequency and biomass removal on soil C and N status and corn yields at three organically managed Minnesota locations. In one experiment, five cutting treatments were applied in nine environments: two, three and four cuts with biomass removal; three cuts with biomass remaining in place; and a no-cut control. In the other experiment, corn was planted following 1-, 2-, 3- or 4-year-old alfalfa stands and a no-alfalfa control. Yield was measured in the subsequent corn crop. In the cutting experiment, the two- and three-cut treatments with biomass removal reduced soil mineral N by 12.6 and 11.5%, respectively, compared with the control. Potentially mineralizable N (PMN) was not generally affected by cutting treatments. The three-cut no-removal increased potentially mineralizable C by 17% compared with the other treatments, but lowered soil total C in two environments, suggesting a priming effect in which addition of alfalfa biomass stimulated microbial mineralization of native soil C. Although both yields and soil mineral N tended to be higher in treatments where biomass remained in place, this advantage was small and inconsistent, indicating that farmers need not forgo hay harvest to obtain the rotational benefits of an alfalfa stand. The lack of overall correlation between corn grain yields and mineral and potentially mineralizable N suggests that alfalfa N contribution was not the driver of the yield increase in the no-removal treatments. Alfalfa stand age had inconsistent effects on fall-incorporated N and soil N and C parameters. Beyond the first year, increased alfalfa stand age did not increase soil mineral N or PMN. However, corn yield increased following older stands. Yields were 29, 77 and 90% higher following first-, second- and third-year alfalfa stands than the no-alfalfa control, respectively. This indicates that alfalfa may benefit succeeding corn through mechanisms other than N contribution, potentially including P solubilization and weed suppression. These effects have been less studied than N credits, but are of high value in organic cropping systems.