Platelet activation plays a key role in the pathogenesis of cardiovascular complications in patients with coronary and cerebrovascular ischemic disease. The most important task in the treatment and prevention such complications is the complete suppression of platelet activity. Inhibition of platelet aggregation is currently a key therapeutic approach in the treatment and prevention of cardiovascular atherosclerotic origin. The development of new and more effective methods for prediction of the response of patients to antiplatelet therapy and its subsequent correction appears to be the most important task of modern physics, biophysics, and medicine. Platelets obtained from patient specimens have been investigated by Surface-enhanced Raman spectroscopy (SERS) with the diode-pumped solid-state laser (λ = 532 nm) on Au/Ti rough surfaces. As a result, a set of optimal experimental parameters for platelets discrimination have been determined. Platelet spectral data for healthy volunteers, healthy volunteers on antiplatelet therapy and individuals with cardiovascular pathology on antiplatelet therapy have been analyzed. We suggest that the obtained results allow the usage of the SERS as a diagnostic tool for determination of both inhibition of platelet aggregation and its structure conformation.
The article describes the results of finite-difference time-domain (FDTD) mathematical modeling of electric field strength distribution near the gold laser-induced periodic surface structures (LIPSS). Both theoretical and experimental results have been described for two fabricated morphologies: round «hill-like» and grating structures. The structures were fabricated by using a femtosecond Yb-fiber laser with a wavelength of λ=1032 nm, pulse duration τ=280 fs, and repetition rate υ=25 kHz. Morphological properties of the surfaces have been investigated by means of scanning electron microscopy (SEM). The plasmonic activity was analyzed by means of the surface-enhanced Raman spectroscopy (SERS) technique. FDTD-calculated electric field values were converted into the electromagnetic field enhancement coefficient and the theoretical SERS intensity. The prospects of the theoretical approach for LIPSS to evaluate optimal field amplification and light scattering parameters has been shown. The presented approach could be applied as a basis for performing the methods of controlled synthesis for LIPPS.
This paper describes a detailed study of the spectral homogeneity of human platelets using Surface-enhanced Raman spectroscopy (SERS). We used a combined approach based on multivariate methods as principal component analysis and pair correlation algorithms to investigate platelets spectral properties. The correlation coefficients for each sample have been calculated, and the average coefficient of determination has been estimated. The high degree of spectral homogeneity inside one probe and between them has been revealed. The prospects of obtained results usage for pathologies based on platelet conformations during cardiovascular diseases have been demonstrated.
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