Background: Pulicaria is a genus of the Asteraceae family, tribe Inuleae, containing 100 species with a distribution from Europe to North Africa to Asia. Objective: In this work, the study is focused on the difference in phytochemical and antibacterial activity of Pulicaria odora harvested in two regions of northern Algeria. Methods: Two maceration extraction methods were used; at room temperature and bath shaker at 37 °C. Total phenolics and flavonoids were carried out using folin ciocalteu and aluminium chloride methods, respectively. Antibacterial activity was evaluated by agar diffusion method on four bacterial strains Listeria innocua, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa. Results: The results found showed that the highest total polyphenol content was 271,8 ± 22,52 mg gallic acid equivalent / g dry matter (mg GAE/g of DM); the best flavonoid content is 51,53 ± 4,42 mg quercetin equivalent / g of dry matter (mg QE/g of DM) harvested in Bejaia. The best level of total polyphenols and flavonoids from the plant harvested in the region of Tizi-Ouzou are 218,23 ± 12.33 mg GAE / g of DM, 54,15 ± 5,98 mg QE / g DM respectively. The two phenolic extracts obtained by the two solvents at a concentration of 70% are more active against S. aureus (17.66 mm for the sample of Bejaia at the concentration 1mg /20µL), while a moderate sensitivity is recorded against other strains for the two samples. Conclusion: Pulicaria odora leaf extract is strong against bacterial infection and can be used as medicinal remedy and in food industry.
: The aim of this study was to evaluate the anticoagulant activity of five marine algae from Bejaia’s coast (Algeria). Higher contents of phenolic compounds were obtained with ethanol for Cystoseira humilis, Halopteris scoparia, Padina pavonica and Rhodomela confervoides (8.55±0.29, 7.84±0.47, 6.41±0.14 and 4.16±0.04 mg CE/g of dw, respectively). Whereas, for Sargassum vulgare, the extraction with acetone showed higher content (3.04±0.071 mg CE/g of dw).The determination of sugar content showed that acetone extract of the five seaweeds was the richest in sugar, this rate increasingly dropped in ethanol extract and the fractions A and B. The red algae Rhodomela confervoides showed a significant anticoagulant activity in ethanol extract and the fractions A, B and C, with an elongation up to 407.97±58.12 s in the fraction C, at a concentration of 10 mg/mL. An anticoagulant activity was observed in the fractions A, B and C of all the brown seaweeds. A procoagulant activity was observed in the fractions A, B of Cystoseira humilis and Sargassum vulgare as well as the fraction B of Padina pavonica with an indeterminate period, at 10 mg/mL.
Background: Asteraceae family, of which Pulicaria genus, plays a remarkable role in the development of drugs used in modern medicine. Leaf extracts of Pulicaria odora harvested in Bejaia were evaluated for their antioxidant and antimicrobial activities. Methods: HCl method at three different wavelengths 280, 320 and 360 nm was carried out for total phenolics content and characterized by ultra-performance liquid chromatography (UPLC) analysis. Antimicrobial activity was evaluated using four bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Listeria innocua) and three molds (Aspergillus flavus, Aspergillus niger and Trichoderma reesei). Four antioxidant tests (ORAC, ABTS, DPPH and FRAP) were performed. Results: The extracts had high total polyphenols (305 mg catechin equivalents [CE]), flavonols (56 mg quercetin equivalents [QE]), tannins (77 mg CE) and tartaric acids (38 mg caffeic acid equivalents [CAE]) per g dry matter contents. UPLC analysis of the 70 % ethanol extracts showed abundant phenolic acids (protocatechuic, chlorogenic and caffeic acids). Pulicaria odora extracts exhibited good antimicrobial activity against bacterial (~18 mm inhibition zones) and molds (11.3-17.3 mm) strains. Conclusion: All extracts displayed good antioxidant activity and effective antimicrobial activity, which allow its use as a conservative agent or therapeutic remedy.
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