Purpose Since December 2019, the coronavirus disease 2019 (COVID-19) epidemic has swept the world, causing widespread burden and increasingly hospitalizations. Researchers from around the world have tried to study the virus and its effect with more precision in various fields. The purpose of this study is to identify levels of anxiety and depression with regard to precautionary for prevention of COVID-19, and to identify the relationship between demographic variables and both depression and anxiety. Methods This was a descriptive cross-sectional study; data were collected by questionnaire via a mobile phone application in the Kurdistan Region of Iraq from 25 March, 2020 to 5 April, 2020. The sample size was 894 after deleting 20 cases because of duplication. The questionnaire consists of three main parts; part one is related to the sociodemographic characteristics of participants, the second and third parts consist of items related to depression and anxiety about COVID-19 using a 5-point Likert Scale (1 = of course no, 2 = no , 3 = normal for me, 4 = yes, and 5 = of course yes). Data was analyzed using SPSS V.25. Results The majority of the participants were from Erbil (58.8%), most of them were male (58.4%); nearly 21.2% preferred quarantine and 41.7% chose curfew as a best way to to avoid being infected by COVID-19. Most of the participants had depression because of people's lack of knowledge about how to protect themselves from the virus (88.14%), while the majority of them had anxiety concerning shopping and contact with infected people (97%) and financial problems (97%). Females had higher rates of COVID-19 depression than did males. There was a significant correlation between age and home setting and anxiety, and a significant association between marital status and level of education and depression. There was no significant association between other variables and depression and anxiety Conclusion The findings of the study indicated that the majority of participants were depressed and had anxiety about COVID-19. There was a significant association between gender and depression and anxiety, while there was no significant association between occupation and income, and depression and anxiety.

Onosma species (Boraginaceae) are well known as medicinal plants due to their wide range of pharmaceutical potential. The present study aims to investigate the anticancer (in vitro) and chemo-protective (in vivo) efficacies of Onosma mutabilis extract (OME) in the azoxymethane (AOM)-induced aberrant crypt foci (ACF) in rats. The in vitro antiproliferative effects of OME were determined on two human tumor cell lines (Caco-2 and HT-29) via MTT assay. The in vivo chemoprotective effects of OME were investigated by performing various biochemical analyses in serum and tissue homogenates of albino rats, along with determining oxidative stress biomarkers. Inflammatory biomarkers of colon, colonic gross morphology (by methylene blue), ACF formation, and colonic histopathology (H & E stain) were determined. The immunohistochemistry of colonic tissues was also assessed by Bax and Bcl-2 protein expression. The results showed that the antitumor activity of OME against Caco-2 and HT-29 colorectal cancer cells ranged between 22.28–36.55 µg/mL. OME supplementation caused a significant drop in the ACF values and improved the immunohistochemistry of the rats shown by up-regulation of Bax and down-regulation of Bcl-2 protein expressions. These outcomes reveal that O. mutabilis may have chemoprotective efficiency against AOM-induced colon cancer represented by the attenuation of ACF formation possibly through inhibition of free radicals, inflammation, and stimulation of the colon antioxidant armory (SOD, CAT, and GPx) and positive regulation of the Nrf2-Keap1 pathway.

The genus Onosma belongs to the Boraginaceae family and contains over 230 species. The present review sheds light on the ethnopharmacology, phytoconstituents, bioactivity, and toxicology of the Onosma species from previous investigations. Furthermore, the paper also highlights the unresolved issues for the future investigations. The review included previous studies of the genus Onosma available from Google Scholar and Baidu Scholar, Science Direct, SciFinder, Wiley Online Library, and Web of Science. Until now, more than 200 chemical compounds have been detected from the genus Onosma, including naphthoquinone (33), flavonoids (30), hydrocarbon (23), phenolic (22), ester (17), alkaloids (20), aromatics (12), carboxylic acid (11), fatty acids (9), terpenoids (10), while the most important ones are rosmarinic, ferulic, protocatechuic, chlorogenic, caffeic, p-coumaric acids, and apigenin. The Onosma species are reported as traditional medicine for wound healing, heart disease, and kidney disorders, while the pharmacological investigations revealed that the extracts and the phytochemicals of Onosma species have different therapeutic properties including antioxidant, enzyme inhibitory, antitumor, hepatoprotective, antiviral, anti-inflammatory, and antimicrobial actions. The summarized knowledge in this review provides valuable ideas for the current and future drug discovery and a motivation for further investigation on the genus Onosma.