Three uncommon morpholine-based fluorescent probes (A, B and C) for pH were prepared by introducing morpholine residues to BODIPY dyes at 4,4’- and 2,6-positions, respectively. In contrast to morpholine-based fluorescent probes for pH reported in literature, these fluorescent probes display high fluorescence in a basic condition while they exhibit very weak fluorescence in an acidic condition. The theoretical calculation confirmed that morpholine is unable to function as either an electron donor or an electron acceptor to quench the BODIPY fluorescence in the neutral and basic condition via photo-induced electron transfer (PET) mechanism because the LUMO energy of morpholine is higher than those of the BODIPY dyes while its HOMO energy is lower than those of the BODIPY dyes. However, the protonation of tertiary amines of the morpholine residues in an acidic environment leads to fluorescence quenching of the BODIPY dyes via d-PET mechanism. The fluorescence quenching is because the protonation effectively decreases the LUMO energy which locates between the HOMO and LUMO energies of the BODIPY dyes. Fluorescent probe C with deep-red emission has been successfully used to detect pH changes in mammalian cells.
First principles calculations based on hybrid density functional theory have been used to study the electronic and geometric properties of armchair silicon nanotubes from (4 4) to (12 12). Full geometry and spin optimizations have been performed without any symmetry constraints with an all electron 3-21G * basis set and the B3LYP functional. The largest silicon nanotube studied has a cohesive energy of 3.47 eV/atom. Atomic hydrogen and oxygen adsorptions on a Si(6 6) tube have been studied by optimizing the distances of the adatoms from both inside and outside the tube. The on-top external site is the most preferred site for hydrogen with an adsorption energy of 6.00 eV and an optimized distance of 1.50 A. For oxygen, the external normal bridge site is the most preferred site with an adsorption energy of 9.68 eV, the optimized distance being 1.66 A. In contrast to some published results, HOMO-LUMO gaps decrease for H adsorption but remain about the same for O adsorption. Radial buckling increases significantly due to H and O adsorptions.
Background: Patellofemoral pain is the leading cause of knee pain in young adults. Magnetic resonance imaging plays an important role in early diagnosis of patellofemoral joint pathology. This study was carried out to evaluate the patellofemoral joint using magnetic imaging resonance and describe various predisposing factors for patellofemoral instability. Methods: The study was carried in Department of Radiology, BPKIHS over a period of six months from February 2020 to August 2020. All patients with clinical diagnosis of patellar instability were included and Magnetic resonance imaging was done using standard knee protocol and findings were noted on structured proforma. Analysis was done using statistical package for the social sciences version 20 applying simple descriptive statistical methods.Results: A total of 60 patients who underwent MRI knee were analyzed, out of which 28(46.7%) patients were male while 32(53.3%) patients were female. 44 patients (58.3%) had various predisposing factors for patellar instability. The commonest predisposing factor was patellar subluxation (73.3%) followed by abnormal trochlear groove angle (58.3%) and patellar translation (increased tibia tubercle trochlear groove distance) (53.3%). Various MRI findings in our study were bone contusion (28 cases,46.7%), joint effusion (36 cases,60%), medial patellofemoral ligament injury (11cases ,18.3%), erosion of patellar cartilage (5 cases,8.3%), femoral cartilage erosion (3 cases,5%),loose bodies (2 cases,3.3%), subchondral edema (3 cases,5%) and meniscus injury (18 cases,30%).Conclusions: Magnetic resonance imaging is not only useful in assessing lesions of the bone, cartilage and ligaments of patellofemoral joint but also enables detection of various predisposing factors for patellofemoral instability.Keywords: Magnetic resonance imaging; patellofemoral instability; patellofemoral joint
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