The assessment scores of the skin lesions and total serum IgE levels of PLE-treated mice (PLE group) were significantly lower than DFE-treated mice (DFE group) on days 7, 14, and 21. On day 31, the serum periostin and thymus and activation-regulated chemokine (TARC) levels in the PLE group were significantly lower than those in the DFE group. Histological analysis of the skin revealed that hyperplasia of the epidermal and dermal layers and infiltration of inflammatory cells (cell infiltration in corium tissues) were suppressed by PLE. Periostin deposition was observed in the skin tissue obtained from the DFE group. Moreover, the CD4+/CD8+ ratio of splenic T cells was suppressed in the PLE group but not in the DFE group.
In this paper, circular cross-section microchannels with 3-D lattice arrangements are designed and fabricated using the Mosquito method in order to construct on-off valves. The 3-D microchannels with on-off valves consist of two types of lines: the flow lines for chemical liquid flow and the control lines to activate the valves. We confirmed that both a circular cross-section and a PDMS with low elastic modulus used as the microchannel material contribute to a valve that can be closed with a lower pressure. Then, we demonstrated liquid flow to evaluate the functionality of the valve. Fluorescein solution was flown into a flow line. We found that the fluorescence intensity decreases at the intersection between the flow and control lines when the flow line is closed by the inflation of the control line, experimentally confirming the functionality of the valve microchannels fabricated via the Mosquito method.
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