Perilla leaf extract prevents atopic dermatitis induced by an extract of Dermatophagoides farinae in NC/Nga mice

Abstract

The assessment scores of the skin lesions and total serum IgE levels of PLE-treated mice (PLE group) were significantly lower than DFE-treated mice (DFE group) on days 7, 14, and 21. On day 31, the serum periostin and thymus and activation-regulated chemokine (TARC) levels in the PLE group were significantly lower than those in the DFE group. Histological analysis of the skin revealed that hyperplasia of the epidermal and dermal layers and infiltration of inflammatory cells (cell infiltration in corium tissues) were suppressed by PLE. Periostin deposition was observed in the skin tissue obtained from the DFE group. Moreover, the CD4+/CD8+ ratio of splenic T cells was suppressed in the PLE group but not in the DFE group.

Lamiaceae: An Insight on Their Anti-Allergic Potential and Its Mechanisms of Action

“…Results demonstrated a promising reduction in serum IgE level in the OVA-sensitized mice with 320 µg of ethanolic extract of P. frutescens leaves and hence amelioration of asthmatic symptoms (Chen et al, 2015). A similar reduction outcome was obtained in the study using Der f ( Dermatophagoides farinae ) mite-induced atopic dermatitis murine model with oral administration of P. frutescens leaves extract (Komatsu et al, 2016). In the latest work by Kamei et al (2017), a new active principle, 8-hydroxy-5,7-dimethoxyflavanone (PDMF) (4) was isolated from the leaves of P. frutescens and demonstrated to have a potent suppressive effect on PCA reaction in anti-DNP IgE-stimulated-BALB/c mice.…”

“…Therefore, the combination of suppressed IL-31 and augmented T-bet protein expression resulted in T H 1/T H 2 balance and hence alleviation of allergic symptoms. Besides, Komatsu et al (2016) suggested that the CD4 + /CD8 + ratio in splenic T lymphocytes obtained from Der f -induced atopic dermatitis NC/Nga mice was decreased from 1.82 ± 0.32% to 1.50 ± 0.07% after receiving treatment of Perilla leaves extract. This suppression is believed to be associated with the T H 1 and T H 2 balance (Komatsu et al, 2016).…”

“…Besides, Komatsu et al (2016) suggested that the CD4 + /CD8 + ratio in splenic T lymphocytes obtained from Der f -induced atopic dermatitis NC/Nga mice was decreased from 1.82 ± 0.32% to 1.50 ± 0.07% after receiving treatment of Perilla leaves extract. This suppression is believed to be associated with the T H 1 and T H 2 balance (Komatsu et al, 2016). Additionally, Liu et al (2013) employed an in vitro assay to investigate the effect of P. frutescens extract on Der p 2-challenged human bronchial epithelial cells BEAS-2B cells.…”

Perilla frutescens: A Rich Source of Pharmacological Active Compounds

“…The anti-dermatitis activity of the aqueous extract of P. frutescens is, possibly, due to the downregulation of matrix metalloproteinase-9 (MMP-9) and IL-31 expression levels, and upregulation of T-bet activity. Komatsu et al [ 76 ] demonstrated that P. frutescens leaf extract prevents atopic dermatitis induced by house dust mite allergens ( Dermatophagoides farina ), in an NC/Nga AD mouse model. Moreover, P. frutescens leaf extract significantly decreased the serum levels of inflammatory markers, such as IgE, periostin, and TARC.…”

A Novel Perilla frutescens (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo

“…P. frutescens has historical importance in Asian medicine, but it is known in modern phytotherapy mainly to treat seasonal allergy symptoms [ 1 ]. In recent years, this species has been investigated in vitro and animal models for the potential role of leaf extracts in atopic dermatitis [ 2 ] and UV-induced skin damage [ 3 ]. The biological effects of P. frutescens preparations have been linked to its peculiar polyphenolic phytocomplex in which rosmarinic acid (RA) has the main role [ 4 ].…”