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Several subunits in the matrix domain of mitochondrial complex I (CI) have been posited to be redox sensors for CI, but how elevated levels of reactive oxygen species (ROS) impinge on CI assembly is unknown. We report that genetic disruption of the mitochondrial NADPH-generating enzyme, isocitrate dehydrogenase 2 (IDH2), in Drosophila flight muscles results in elevated ROS levels and impairment of assembly of the oxidative phosphorylation system (OXPHOS). Mechanistically, this begins with an inhibition of biosynthesis of the matrix domain of CI and progresses to involve multiple OXPHOS complexes. Despite activation of multiple compensatory mechanisms, including enhanced coenzyme Q biosynthesis and the mitochondrial unfolded protein response, ferroptotic cell death ensues. Disruption of enzymes that eliminate hydrogen peroxide, but not those that eliminate the superoxide radical, recapitulates the phenotype, thereby implicating hydrogen peroxide as the signaling molecule involved. Thus, IDH2 modulates the assembly of the matrix domain of CI and ultimately that of the entire OXPHOS.

Salinity intrusion is a global concern in relation to sustainable agricultural practices. About three-fourths of the coastal area in Bangladesh is affected by different levels of salinity. This study was conducted to observe the effects of NaCl-salinity on tomato (Lycopersicon esculentum Mill.) plants in a pot experiment. Morphological properties and yield of five varieties of Bangladesh Agricultural Research Institute tomato (BARI-T 1, BARI-T 2, BARI-T 3, BARI-T 4, and BARI-T 5) plants were exposed to NaCl-salinity (2, 4, 6 and 8 dS/m) through irrigation. Morphological properties such as number of leaves, leaf length, leaf width, number of days of flowering, number of flowers and number of branches were observed. Results showed that morphological properties of all studied tomato plants were affected by increasing NaCl-salinity, except number of days of flowering, and yield was reduced compared to the control group (tap water; 0.043 dS/m). The post-harvest pot soil was also affected by NaCl-salinity irrigation. This study can be useful in selecting salt-tolerant varieties in saline-affected areas.

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