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Funding informationScience Achievement Scholarship of Thailand (SAST); Mae Fah Luang University (MFU) Copper(II) (Cu 2+ ) ion-imprinted polymers were prepared by suspension polymerization using 2.5 mmol of copper(II) sulfate pentahydrate as the template ion, 5 mmol of 4-vinylpyridine and methacrylic acid as functional monomers, 20 mmol of ethylene glycol dimethacrylate as the cross-linker, 1 mmol of 1,1′-azobis (cyclohexanecarbonitrile) as the initiator in the porogen of 10 mL of toluene, and hydroxypropyl methyl cellulose as a stabilizer in the polymerization reaction. The obtained polymers were used to quantify Cu 2+ ion by using flame atomic absorption spectroscopy. Chemical structure, morphology, crystalline structure, and surface area of polymers were investigated by Fourier transform infrared spectroscopy, scanning electron microscope, X-ray diffraction, and Brunauer-Emmett-Teller respectively. The diameters of the synthesized polymer particles were about 250 nm. The Cu 2+ ion-imprinted polymers illustrated good recognition property and selective adsorption ability with Cu 2+ higher than Mn 2+ and Fe 2+ . Thus, this work could be applied as a sorbent from the enrichment of Cu 2+ ions in analytes.

The prednisolone molecularly imprinted polymer nanoparticles (prednisolone-MIPs) were prepared via precipitation polymerization. The synthetic conditions were performed by optimizing of functional monomers (acrylamide and methacrylic acid) and crosslinkers (ethylene glycol dimethacrylate and pentaerythritol triacrylate) with defined the synthetic ratio of template molecule:functional monomer:crosslinker as 1:7:30. The acquired MIPs were studied for their binding quantitation by kinetic adsorption analysis. Two of four synthetic conditions reached an equilibrium binding capacity at 960 minutes. In addition, the equilibrium adsorption, kinetic models, adsorption isotherms, and molecular selectivity were also evaluated in this study.The pseudo-second order was the best fitted kinetic model with good agreement on Langmuir isotherm of both optimum conditions. The molecular recognition of prednisolone-MIPs was further carried out to demonstrate the selectivity performance and found that prednisolone-MIPs had a good recognition to target prednisolone rather than structural analogue.

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Bacterial fruit blotch (BFB) is a bacterial disease that devastates Cucurbitaceae crops worldwide, causing significant economic losses. Currently, there is no means to treat or control the disease. This study focused on exploring the antibacterial properties of endophytic fungi against Acidovorax citrulli (Aac), the causative agent of BFB. Based on disc diffusion, time kill and MIC microdilution broth assays, four endophytes showed promise in controlling Aac. Nonetheless, only one strain, Neocosmospora sp. MFLUCC 17-0253, reduced the severity of disease on watermelon and melon seedlings up to 80%. Structure analysis revealed production of several compounds by the fungus. Three of these secondary metabolites, including mixture of 2-methoxy-6-methyl-7-acetonyl-8-hydroxy-1,4-maphthalenedione and 5,8-dihydroxy-7-acetonyl-1,4-naphthalenedione, anhydrojavanicin, and fusarnaphthoquinones B exhibited antagonistic activity against Aac. The chemical profile data in planta experiment analyzed by LC-Q/TOF-MS suggested successful colonization of endophytic fungi in their host plant and different metabolic profiles between treated and untreated seedling. Biofilm assay also demonstrated that secondary metabolites of Neocosmospora sp. MFLUCC 17-0253 significantly inhibited biofilm development of Aac. To the best of our knowledge, secondary metabolites that provide significant growth inhibition of Aac are reported for the first time. Thus, Neocosmospora sp. MFLUCC 17-0253 possesses high potential as a biocontrol agent for BFB disease.

Novel copper (II) ion‐imprinted polymers (Cu‐IIPs) were synthesized by bulk polymerization using 1 mmol of copper (II) sulfate pentahydrate as the template ion, 1 mmol of ethylenediaminetetraacetic acid (EDTA) as the ligand, and 4 mmol of 4‐vinyl pyridine (4‐VP) or methacrylic acid (MAA) as the functional monomer dissolved in a porogen of 20 mL of methanol. Polymerization was performed with 20 mmol of pentaerythritol triacrylate (PETA) as the crosslinker and 1 mmol of benzoyl peroxide as the initiator. The effect of pH, time, initial concentration of Cu2+, and selectivity tests on the adsorption capacity were investigated using flame atomic absorption spectroscopy (FAAS). The prepared polymers were characterized by Fourier transform infrared spectroscopy (FTIR), Brunauer–Emmett–Teller (BET) analysis, scanning electron microscopy (SEM) and X‐ray diffraction (XRD). Adsorption capacity of Cu‐IIPs prepared with MAA increased from 0.794 to 2.163 mg g−1 at pH 6 within 24 hours, while Cu‐IIPs from MAA had high recognition ability with better recognition of Cu2+ than other ions. Thus, these Cu‐IIPs demonstrated potential application as an adsorbent for efficient removal of Cu2+ from aqueous solutions.

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