Rapid antioxidant capacity screening in herbal extracts using a simple flow injection-spectrophotometric system

Mrazek, Nookrai; Watla‐iad, Kanchana; Deachathai, Suwanna; Suteerapataranon, Siripat

doi:10.1016/j.foodchem.2011.10.066

Cited by 17 publications

(7 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To determine the total antioxidant capacity of GE samples, DPPH assays were performed by using the method introduced by Mrazek et al . 36 with slightly modification. In this study, the total antioxidant activities of the GE samples were assessed by the on-line UV spectroscopic DPPH method, where ascorbic acid equivalent (ASAE) values were measured as shown in Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…However, the batch DPPH assay has limitations such as high consumption, labour intensive and time consuming. Flow injection analysis (FIA) was ideally suited for automated analysis and had been successfully applied to rapid screening of food and pesticide 34–36 . As a kind of homologous medicine and food, licorice has high research value.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A strategy for qualitative and quantitative profiling of glycyrrhiza extract and discovery of potential markers by fingerprint-activity relationship modeling

Zhang

Wang

Yang

et al. 2019

Sci Rep

View full text Add to dashboard Cite

This study was to evaluate the quality consistency of glycyrrhiza extract and to explore the possible anti-oxidant components in combination with chromatographic fingerprint and bioactivity evaluation. Characteristic fingerprints of glycyrrhiza extract samples from different sources were generated by high performance liquid chromatography with diode array detector (HPLC-DAD) and evaluated using hierarchical clustering and similarity analysis. Compared with the conventional qualitative similarity evaluation method, the averagely linear quantified fingerprint method had an important quantitative similarity parameter supported by quantitative analysis, which was recommended in the fingerprint evaluation. Antioxidant activities of the glycyrrhiza extract samples were determined by DPPH (2, 2-diphenyl-1-picryldrazyl) radical scavenging assays. In addition, the fingerprint-efficacy relationship was investigated by the chemical fingerprints and the anti-oxidant activities utilizing partial least squares model, which was capable of exploring and discovering the bioactive components of glycyrrhiza extracts. Therefore, the present study provided a powerful strategy to evaluate the holistic quality consistency of medicinal plant.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A strategy for qualitative and quantitative profiling of glycyrrhiza extract and discovery of potential markers by fingerprint-activity relationship modeling

Zhang

Wang

Yang

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The assays of DPPH radical scavenging activity were monitored by UV according to Mrazek et al. with slight modification. Total antioxidant capacity was measured on an Agilent 1100 HPLC series (Agilent Technology, USA), equipped with a UV‐vis DAD over the range 190–600 nm.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the quality consistency of powdered poppy capsule extractive by an averagely linear‐quantified fingerprint method in combination with antioxidant activities and two compounds analyses

Zhang

Sun

Hou

et al. 2017

J of Separation Science

View full text Add to dashboard Cite

A novel averagely linear-quantified fingerprint method was proposed and successfully applied to monitor the quality consistency of alkaloids in powdered poppy capsule extractive. Averagely linear-quantified fingerprint method provided accurate qualitative and quantitative similarities for chromatographic fingerprints of Chinese herbal medicines. The stability and operability of the averagely linear-quantified fingerprint method were verified by the parameter r. The average linear qualitative similarity SL (improved based on conventional qualitative "Similarity") was used as a qualitative criterion in the averagely linear-quantified fingerprint method, and the average linear quantitative similarity PL was introduced as a quantitative one. PL was able to identify the difference in the content of all the chemical components. In addition, PL was found to be highly correlated to the contents of two alkaloid compounds (morphine and codeine). A simple flow injection analysis was developed for the determination of antioxidant capacity in Chinese Herbal Medicines, which was based on the scavenging of 2,2-diphenyl-1-picrylhydrazyl radical by antioxidants. The fingerprint-efficacy relationship linking chromatographic fingerprints and antioxidant activities was investigated utilizing orthogonal projection to latent structures method, which provided important pharmacodynamic information for Chinese herbal medicines quality control. In summary, quantitative fingerprinting based on averagely linear-quantified fingerprint method can be applied for monitoring the quality consistency of Chinese herbal medicines, and the constructed orthogonal projection to latent structures model is particularly suitable for investigating the fingerprint-efficacy relationship.

show abstract

“…3.3.4 Antioxidant activity conditions. The DPPH radical scavenging activity assays were performed according to Mrazek et al 15 with slight modication, as shown in Fig. 1A.…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

“…In this situation, ow-based methods, such as ow injection analysis (FIA), multisyringe FIA, and sequential injection analysis, have been proposed and even successfully applied in automated analysis for rapid screening purposes. [13][14][15] On the basis of FIA, UV spectroscopic ngerprinting is preferred to monitor the TCM/ HM quality, owing to its apparent analytical advantages, such as low experimental expenses, simple sample preparation, and short measurement time. It is common knowledge that UV spectra can display the features of unsaturated bonds information, such as for n-s*, n-p*, and p-p*, qualitatively and quantitatively; 16 therefore, UV spectroscopic ngerprinting in combination with chemometric methods can be used for monitoring the TCM/HM quality in a straightforward, rapid, and effective manner.…”

Section: Introductionmentioning

confidence: 99%

UHPLC-ESI-Q-TOF-MS/MS analysis, antioxidant activity combined fingerprints for quality consistency evaluation of compound liquorice tablets

et al. 2018

View full text Add to dashboard Cite

show abstract

Rapid antioxidant capacity screening in herbal extracts using a simple flow injection-spectrophotometric system

Cited by 17 publications

References 20 publications

A strategy for qualitative and quantitative profiling of glycyrrhiza extract and discovery of potential markers by fingerprint-activity relationship modeling

A strategy for qualitative and quantitative profiling of glycyrrhiza extract and discovery of potential markers by fingerprint-activity relationship modeling

Evaluation of the quality consistency of powdered poppy capsule extractive by an averagely linear‐quantified fingerprint method in combination with antioxidant activities and two compounds analyses

UHPLC-ESI-Q-TOF-MS/MS analysis, antioxidant activity combined fingerprints for quality consistency evaluation of compound liquorice tablets

Contact Info

Product

Resources

About