In this study, the RAPD (Random Amplified Polymorphic DNA) technique was employed for determination of the components in an Ayurvedic herbal prescription, Rasayana Churna. One-hundred-and-twenty decamer oligonucleotide primers were screened in the RAPD analysis to identify three Ayurvedic medicines, dried stem of Tinospora cordifolia, dried fruit of Emblica officinalis and dried fruit of Tribulus terestris, the Ayurvedic prescription. Primer OPC-6 simultaneously generated three distinct amplicons, each specific to one component. The marker with 600 bp is specific to Tinospora cordifolia; the marker 500 bp is specific to Emblica officinalis and the remaining marker >1000 bp was present in Tribulus terestris. Presence of three herbal medicines was determined when RAPD reaction with OPC-6 was performed. The technique was proved to contribute to the identification of components in Ayurvedic herbal preparation and thus helping to serve as a complementary tool for quality control.
Aims: Malnutrition among all ages is still a persistent problem in India, especially in areas where the poor largely depend on rice and wheat staples with limited access to diverse diets using underutilized foods. This study was conducted to nutritionally enhance traditional food products like roti and lapsi utilizing suitable composite flours based on amaranth, soybean and wheat without affecting their sensory quality.
Study Design: Different combinations of amaranth, soybean and wheat flours were made to suit the quality characteristics of roti and lapsi.
Place and Duration of Study: Department of Foods and Nutrition, G. B. Pant University of Agriculture & Technology, Pantnagar (India), between January and June 2016.
Methodology: The sensory evaluation of food products and estimation of nutritional composition of composite flours was done using standard procedures.
Results: The composite flours having 25% amaranth, 15% soybean and 60% wheat flour and 25% amaranth, 10% soybean and 65% wheat flour were found to be most acceptable sensorially and were significantly superior to their control counterparts for protein, ash, fibre, carbohydrate calcium and iron content (p=.05).
Conclusion: Roti made from amaranth and soybean incorporated composite flours with better protein quality and low available carbohydrates and physiological energy almost same as control would be better diet alternative to diabetic and overweight patients whereas lapsi may be effectively used as supplementary food. Many other traditional food products like laddoo, halwa, puri, parantha, burfi etc. may also be made from such composite flours.
The present communication reports an efficient in vitro plantlet regeneration protocol for endemic umbellifer Centella asiatica (L.) urban via callus mediated organogenesis from leaf and stem explants. The plant is pharmacologically very important and its consumption as underutilized green leafy vegetable affluent in micronutrients is communally conscientious for its threatened status. Therefore, there is an importunate need to preserve its germplasm so that pharmacologically active constituent can be made available all over the year without causing loss of species from wild. Optimum callusing was observed in MS + benzylaminopurine (BAP, 0.5 mg/l) + α-napthalene acetic acid (NAA, 0.3 mg/l) in both leaf and stem explants with callus induction frequency 75 and 83.33%, respectively. For shooting, MS + BAP (0.5 mg/l) in leaf and MS + BAP (0.75 mg/l) in stem derived callus were found to be most efficient. Rooting of in vitro raised shoots was best induced on full strength MS media supplemented with indole-3-butyric acid (IBA, 0.5 mg/l). The regenerated plants were acclimatized in controlled environment and successfully transferred in field condition displaying normal development.
In the modern era of science, nanotechnology has the ability to circumvent numerous disadvantages of conservative healing preparations. Important progress has been made towards the use of tailored nanomaterials (NMs) to treat the cancer with efficiency, specificity, and high sensitivity. Tailored NMs are operationalized with precise ligands that can predictably target the cancer cells and deliver encapsulated payloads meritoriously. Moreover, NMs can also be deliberated to increase the drug loading, controlled release, improved half-life, and selective distribution by altering their size, surface chemistry, composition, and morphology. The conservative cancer treatments have provoked the event and applications of nanomaterials. The emerging evidence suggests that nanomedicines will provide the next-generation stages for anticancer remedies.
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