In this study, the RAPD (Random Amplified Polymorphic DNA) technique was employed for determination of the components in an Ayurvedic herbal prescription, Rasayana Churna. One-hundred-and-twenty decamer oligonucleotide primers were screened in the RAPD analysis to identify three Ayurvedic medicines, dried stem of Tinospora cordifolia, dried fruit of Emblica officinalis and dried fruit of Tribulus terestris, the Ayurvedic prescription. Primer OPC-6 simultaneously generated three distinct amplicons, each specific to one component. The marker with 600 bp is specific to Tinospora cordifolia; the marker 500 bp is specific to Emblica officinalis and the remaining marker >1000 bp was present in Tribulus terestris. Presence of three herbal medicines was determined when RAPD reaction with OPC-6 was performed. The technique was proved to contribute to the identification of components in Ayurvedic herbal preparation and thus helping to serve as a complementary tool for quality control.
A simple, sensitive, and reproducible high-performance thin-layer chromatographic method has been established for simultaneous analysis of trigonelline and 4hydroxyisoleucine from fenugreek seeds (Trigonella foenum-graceum). Chromatography was performed on silica gel GF 254 with n-butanol-methanol-acetic acid-water 4:1.5:1:1 (v/v) as mobile phase. Densitometric scanning of the plates at 266 nm was used for analysis of trigonelline, and plates were scanned at 395 nm after derivatisation with ninhydrin reagent for analysis of 4-hydroxyisoleucine. The method was validated for specificity, precision (intraday and interday), accuracy, and robustness. Response was a linear function of the concentration of standard solutions in the range 100 to 1000 ng per band and 50 to 500 ng per band, respectively, with correlation coefficients of 0.9992 ± 0.04 and 0.9986 ± 0.06, for trigonelline and 4-hydroxyisoleucine. The accuracy of the method was checked by determination of recovery at three different levels; average percentage recovery was 99.39 ± 0.34 for trigonelline and 99.03 ± 0.26 for 4-hydroxyisoleucine. The method was found to be simple, precise, specific, sensitive, and accurate and can be used for routine quality control of fenugreek seeds and also for quantitative analysis of these marker compounds in processed fenugreek and its marketed preparations.
Sandalwood (Santalum album L.) is one of the most famous and widely used plants in perfumery and cosmetics. Apart from perfumery and cosmetics uses, sandalwood also has a wide range of pharmacological activities and the plant can be considered as one of the important medicinal plants. Since last two decades, this plant has been studied extensively but still there is lot of scope to exploit full potential of uses of sandalwood for mankind. Various studies report that of effects sandalwood oil is responsible for various effects ranging from antibacterial to anticancer. It also shows prominent activity in various skin diseases. There are few toxicological studies on sandalwood. It is necessary to summaries all activities reported about this plant. This review consolidates different reported activities of sandalwood plant as well as its oil.
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