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Pristine aluminum (Al) has received great deal of attention on fabrication of nanoporous anodized alumina (NAA) with arrays of nanosized uniform pores with controllable pore sizes and lengths by the anodization process. There are many applications of NAA in the field of biosensors due to its numerous key factors such as ease of fabrication, high surface area, chemical stability and detection of biomolecules through bioconjugation of active molecules, its rapidness, and real-time monitoring. Herein, we reviewed the recent trends on the fabrication of NAA for high sensitive biosensor platforms like bare sensors, gold coated sensors, multilayer sensors, and microfluidic device supported sensors for the detection of various biomolecules. In addition, we have discussed the future prospectus about the improvement of NAA based biosensors for the detection of biomolecules.

A highly sensitive and efficient rhodamine 6G based probe (P3) was synthesized for the real‐time sensing of F− and Cu2+ ions. The probe P3 was able to show significant color change from brown to pink in the presence of F− and yellow to orange in presence of Cu2+ ions. The UV‐vis studies confirm that the probe P3 was able to detect F− and Cu2+ ions over other ions. Further, UV‐vis titration studies were carried out to understand the detection mechanism and stoichiometry between P3: F− and P3: Cu2+ ions. 1H‐NMR titration was performed to confirm the F− ion binding to probe P3, where, deprotonation of hydrazone proton (‐C=N‐NH‐) was observed. In addition, the limit of detection for P3:F− and P3:Cu2+ ions were found to be 4.02 μM and 1.64 μM respectively which is useful for practical applications. Lastly, probe P3 was used to detect and quantify the F− ions present in a commercially available mouthwash product. Furthermore, P3 was adsorbed on the surface of silica microsphere to detect Cu2+ ions. Interestingly, the material could visually detect 1 ppm of Cu2+ ions which is lower than the permissible limit.

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