The main idea in this research used quercetin (QT) as a stabilizing agent to prepare CuO nanoparticle (NPs). QT is a pigment which exists from plants, fruits, and in some medicines. It contains numerous hydroxyl groups, works as a complexing agent and a self-assembly agent. The prepared CuO-NPs were characterized by Fourier transformed infrared (FT-IR), X-ray diffraction (XRD), scanning and transmission electron microscopy (SEM and TEM), and energy-dispersive X-ray analysis (EDX). The structure investigation confirm that the CuO-NPs were polycrystalline nature having a monoclinic crystalline form and showed (111̅ ) and (111) preferential orientations. The estimated particle sizes by XRD, TEM, and SEM ranged from 12.03 to 15.3 nm. Surface analysis displayed that homogeneously distributed CuO-NPs with a spherical shape. On the other hand, synthesized CuO-NPs were tested for anticancer activity on the human breast. The infected cell (MCF-7) uses MTT (3-(4,5-dimethyl-2-triazolyl)-2,5-diphenyl-2tetrazolium bromide) test. The inferred CuO-NPs clarify high anticancer cytotoxicity of the (MCF-7) with an IC50 μM value of 57.6 and 42.8 µg/mL for 24 and 72 h respectively.
ABSTRACT. For the first time, silver nanoparticles (AgNPs) were fabricated with graphite pencil electrode (GPE) by immersing a bare hard black pencil (HB pencil) in AgNO3 solution mixed with extract Quercus infectoria solution, followed by placing directly against to UV lamp (254 nm) and kept for 30 min. Silver modified-GPEs were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive X-ray spectrometer (EDX). The silver nanoparticles fabricated with graphite pencil electrode (AgNPs–GPE) showed excellent selectivity to chloride ion, as well as excellent reproducibility, together with it has several advantages such as sensitivity, simplicity, low cost, green, and fast response. The maximum oxidation cyclic voltammogram current of the AgNPs-PGE was found at 160 mV. The linear range of chloride ions detection is between (0.8-52 mmol L-1), and the limit of detection (LOD) of chloride ions is 0.041 mmol L−1. Finally, the Silver fabrication electrode was used to determine the chloride ion concentration in drugs, such as phenylephrine, metformin, procaine, and dopamine formulated with hydrochloride, and satisfactory results were obtained.
KEY WORDS: Electrochemical sensor, Cyclic voltammetry, Pencil graphite electrode, Chloride ion
Bull. Chem. Soc. Ethiop. 2023, 37(2), 491-503.
DOI: https://dx.doi.org/10.4314/bcse.v37i2.17
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