Long non-coding RNAs (lncRNAs) serve a crucial role in every aspect of cell biological functions as well as in a variety of diseases, including cardiovascular disease, cancer and nervous system disease. However, the differential expression profiles of lncRNAs in Marfan syndrome (MFS) have not been reported. The aim of the present study was to identify potential target genes behind the pathogenesis of MFS by analyzing microarray profiles of lncRNA in aortic tissues from individuals with MFS and normal aortas (NA). The differentially expressed lncRNA profiles between MFS (n=3) and NA (n=4) tissues were analyzed using microarrays. Bioinformatics analyses were used to further investigate the candidate lncRNAs. Reverse transcription-quantitative (RT-qPCR) was applied to validate the results. In total, the present study identified 294 lncRNAs (245 upregulated and 49 downregulated) and 644 mRNAs (455 upregulated and 189 downregulated) which were differential expressed between MFS and NA tissues (fold change ≥1.5; P<0.05). Gene Ontology enrichment analysis indicated that the differentially expressed mRNAs were involved in cell adhesion, elastic fiber assembly, extracellular matrix (ECM) organization, the response to virus and the inflammatory response. Kyoto Encyclopedia of Gene and Genomes pathway analysis indicated that the differentially expressed mRNAs were mainly associated with focal adhesion, the ECM-receptor interaction, the mitogen-activated protein kinase signaling pathway and the tumor necrosis factor signaling pathway. The lncRNA-mRNA coexpression network analysis further elucidated the interaction between the lncRNAs and mRNAs. A total of five lncRNAs (uc003jka.1, uc003jox.1, X-inactive specific transcript, linc-lysophosphatidic acid receptor 1 and linc-peptidylprolyl isomerase domain and WD repeat containing 1) with the highest degree of coexpression were selected and confirmed using RT-qPCR. In the present study, expression profiles of lncRNA and mRNA in MFS were revealed using microarray analysis. These results provided novel candidates for further investigation of the molecular mechanisms and effective targeted therapies for MFS.
Plasma bacteria decontamination devices for the inner surface of medical tubes have to consider more practical application problems, such as the processing compatibility for medical tubes of variable scales, a reasonable and effective design model for plasma electrode parameters, and so on. This paper proposes a novel multisegment‐casing ring‐ring jet (MSC‐RRJ) without embedding the electrode into the target tube, obtaining the variable tube length processing capability. And through establishing an effective design model based on electric field distribution properties, ensuring the electric field strength margin in each segment of MSC‐RRJ to achieve the processing capability of variable tube diameters. A prototype device is developed based on the proposed MSC‐RRJ electrode and design method being verified by killing Staphylococcus aureus and Escherichia coli.
To conduct an in‐depth analysis of the bursting of coal slime in its early stage of combustion, a high‐speed camera was used to film the bursting process in a horizontal tube furnace. Three different types of coal slime with water contents of 5%, 10%, 15%, and 20% under different temperatures of 750°C, 850°C, and 950°C were tested. The behavior of coal slime bursting and the influence of water content and temperature were obtained. It appears that there is a critical water content and a deduced range to determine primary bursting intensity. The bursting intensity index (BII) increases with furnace temperature and water content. So a comprehensive consideration of temperature and water content is crucial for industrial design. To investigate influence induced by the bursting, analysis of the micromorphology and pore structure changes of the coal slime samples before and after bursting at 850°C has been done and shows that the coal slime structure after bursting changes greatly compared with the original state of the coal slime. The calculation results of the fractal dimension show that the pore structure of coal slime after bursting becomes more complicated, and the portion of pores in the coal slime generally increases after bursting.
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