BackgroundTIR1-like proteins act as auxin receptors and play essential roles in auxin-mediated plant development processes. The number of auxin receptor family members varies among species. While the functions of auxin receptor genes have been widely studied in Arabidopsis, the distinct functions of cucumber (Cucumis sativus L.) auxin receptors remains poorly understood. To further our understanding of their potential role in cucumber development, two TIR1-like genes were identified and designated CsTIR1 and CsAFB2. In the present study, tomato (Sonanum lycopersicum) was used as a model to investigate the phenotypic and molecular changes associated with the overexpression of CsTIR1 and CsAFB2.ResultsDifferences in the subcellular localizations of CsTIR1 and CsAFB2 were identified and both genes were actively expressed in leaf, female flower and young fruit tissues of cucumber. Moreover, CsTIR1- and CsAFB2-overexpressing lines exhibited pleotropic phenotypes ranging from leaf abnormalities to seed germination and parthenocarpic fruit compared with the wild-type plants. To further elucidate the regulation of CsTIR1 and CsAFB2, the role of the miR393/TIR1 module in regulating cucumber fruit set were investigated. Activation of miR393-mediated mRNA cleavage of CsTIR1 and CsAFB2 was revealed by qPCR and semi-qPCR, which highlighted the critical role of the miR393/TIR1 module in mediating fruit set development in cucumber.ConclusionOur results provide new insights into the involvement of CsTIR1 and CsAFB2 in regulating various phenotype alterations, and suggest that post-transcriptional regulation of CsTIR1 and CsAFB2 mediated by miR393 is essential for cucumber fruit set initiation. Collectively, these results further clarify the roles of cucumber TIR1 homologs and miR393 in regulating fruit/seed set development and leaf morphogenesis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-017-1075-6) contains supplementary material, which is available to authorized users.
In this paper, we report on the results of an investigation into inter-decadal changes in moisture transport and divergence in East Asia for the two periods 1980–2001 and 1958–1979. The aim is to explore the mechanism of summer rainfall change in the region after abrupt changes. The relevant changes are calculated using ERA-40 daily reanalysis datasets. The results show that both stationary and transient eddy moisture transports to the Chinese mainland have declined since the abrupt change in atmospheric general circulation in the late 1970s, leading to more rainfall in South China and less in the North. The anomalous rainfall pattern coincides well with anomalous large-scale moisture divergence in the troposphere, of which stationary-wave or monsoon transport is dominant, in comparison with the contribution of the transient eddies. Furthermore, their divergences are found to be in opposite phases. In addition, meridional divergence is more important than its zonal counterpart, with an opposite phase in East Asia. Abnormal zonal moisture convergences appear in northwestern and northeastern parts of China, and are related to the excess rainfalls in these regions.An increase in transient eddy activity is one of the major mechanisms for excess rainfall in northern Xinjiang. Consequently, the anomalous rainfall pattern in East Asia results from a decline of the East Asian monsoon after the abrupt change, while the rainfall increase in northwestern China involves anomalies of both stationary waves and transient eddies on boreal westerly over the mid- and high latitudes.
BackgroundParthenocarpy is an excellent agronomic trait that enables crops to set fruit in the absence of pollination and fertilization, and therefore to produce seedless fruit. Although parthenocarpy is widely recognized as a hormone-dependent process, hormone-insensitive parthenocarpy can also be observed in cucumber; however, its mechanism is poorly understood. To improve the global understanding of parthenocarpy and address the hormone-insensitive parthenocarpy shown in cucumber, we conducted a physiological and proteomic analysis of differently developed fruits.ResultsPhysiological analysis indicated that the natural hormone-insensitive parthenocarpy of ‘EC1’ has broad hormone-inhibitor resistance, and the endogenous hormones in the natural parthenocarpy (NP) fruits were stable and relatively lower than those of the non-parthenocarpic cultivar ‘8419 s-1.’ Based on the iTRAQ technique, 683 fruit developmental proteins were identified from NP, cytokinin-induced parthenocarpic (CP), pollinated and unpollinated fruits. Gene Ontology (GO) analysis showed that proteins detected from both set and aborted fruits were involved in similar biological processes, such as cell growth, the cell cycle, cell death and communication. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that ‘protein synthesis’ was the major biological process that differed between fruit set and fruit abortion. Clustering analysis revealed that different protein expression patterns were involved in CP and NP fruits. Forty-one parthenocarpy-specialized DEPs (differentially expressed proteins) were screened and divided into two distinctive groups: NP-specialized proteins and CP-specialized proteins. Furthermore, qRT-PCR and western blot analysis indicated that NP-specialized proteins showed hormone- or hormone-inhibitor insensitive expression patterns in both ovaries and seedlings.ConclusionsIn this study, the global molecular regulation of fruit development in cucumber was revealed at the protein level. Physiological and proteomic comparisons indicated the presence of hormone-independent parthenocarpy and suppression of fruit abortion in cucumber. The proteomic analysis suggested that hormone-independent parthenocarpy is regulated by hormone-insensitive proteins such as the NP-specialized proteins. Moreover, the regulation of fruit abortion suppression may be closely related to protein synthesis pathways.Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4290-5) contains supplementary material, which is available to authorized users.
This paper aims at investigating climate integrated column moisture transport (MT in brief hereafter) over the troposphere for 1971~2000 and the relationship between the MT divergence and seasonal precipitation in East Asia. The result made from ERA‐40 shows that the spatial pattern of the precipitation is approximately coincided with large‐scale MT divergence. A further decomposition manifests that the monthly‐wind moisture transport (MMT) is the dominant role for seasonal divergence patterns. The MMT convergence areas approximately fit the big precipitation areas, while its divergence almost covers the arid and semi‐arid zones in Northwest China and Mongolia. The transient eddy moisture transport (EMT) is about ten times smaller than MMT's in magnitude and plays a key role in the MT northward during the non‐monsoon period. Usually, the EMT divergence is of inverse phase to MMT's, suppressing the MMT impact on precipitation in summer monsoon zones, and it is the major one that supplies water vapor to the precipitation in arid and semi‐arid zones. However, their divergences are compatible and overlapped in phase around the phase transition belt of the MT divergence. It turns out that large‐scale precipitation characteristics greatly rely on the competition of the MMT and EMT, being the physical cause that leads to a specific pattern of the large‐scale precipitation. Besides, the mechanism of regional climate formation also implies possible mechanism of regional precipitation change. A case study shows that the severe floods in both the Yangtze River basin and Songhuajiang‐Nenjiang River basin in 1998 were resulted from anomalous MMT convergence with the monsoon MT, while both the MMT and EMT divergences led to anomalous dry climate in the north and the northwest of China. In addition, it is the strong EMT divergence resulting in significant rainfall reduction in south part of North China and the southeast coast of China. One could also make a further investigation on the causes that lead to the anomalies of the MMT or the EMT, or both. This is a new way for exploring the mechanism of regional climate change.
This paper focuses on the mechanism of the trend and oscillation in precipitation records in northwestern China over the past 60 years. The results from regression and orthogonal wavelet decomposition show a clearly ascending in annual precipitation since 2000, which is contributed mostly by significant increase in autumn and winter precipitations, while almost no trend can be identified in spring and summer time, which shows a weak descending in non-linear form. This may be in connection with different warming rates in seasons. There is a significant anti-correlation between the precipitation and the North Atlantic oscillation (NAO) on the inter-annual scale. About 80% of their variances concentrate on inter-annual and multi-decadal scales. The multi-decadal components of NAO index have a phase leading about 10 years to corresponding precipitation, about 4-5 years leg for AMO. Hence, NAO index can be used as an indicator for inter-decadal change of the precipitation. Other results from combination-difference analysis show that the NAO in extreme negative phase could lead to the precipitation increase in central Asia and northwestern China, owing to increase in eastward water-vapor transport from south Europe to Northwestern China, which causes an increase in column water vapor content in the above areas, and meanwhile the transient eddy activity becomes intensified in the areas or north with a further south route moving from Europe, Central Asia throughout northwestern China. On the contrary, a dry climate might appear in the areas when meet extreme positive phase of NAO. Thus, NAO's oscillation is one of the most important mechanisms for inter-annual change in precipitation of northwestern China.
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